{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:57:11Z","timestamp":1789811831615,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T00:00:00Z","timestamp":1151625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aims:\u2002<\/jats:bold> Susceptibility to clarithromycin of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H.\u2003pylori<\/jats:italic>) is caused by single nucleotide polymorphisms (SNPs) of the 23SrRNA gene. Allele specific primer\u2010polymerase chain reaction (ASP\u2010PCR) is one of the methods for determining SNPs, which can measure SNPs easily within a short period by PCR amplification alone without digestion with restriction enzymes. The aim of the present study was to develop the ASP\u2010PCR assay for determining SNPs at positions 2142 and 2143 of the 23S rRNA gene of <jats:italic>H.\u2003pylori<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> In total, 112 patients with <jats:italic>H.\u2003pylori<\/jats:italic> infection based on positive results of a rapid urease test (RUT) were enrolled in the study. Thirty\u2010five had failed to eradicate <jats:italic>H.\u2003pylori<\/jats:italic> by a clarithromycin\u2010based regimen. DNA was extracted from the RUT\u2010positive gastric tissue samples. SNPs from adenine to guanine at positions 2142 and 2143 of the 23S rRNA of <jats:italic>H.\u2003pylori<\/jats:italic> (A2142G and A2143G) were determined by the ASP\u2010PCR method. Minimum inhibitory concentrations (MICs) of clarithromycin for <jats:italic>H.\u2003pylori<\/jats:italic> were also measured.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Forty\u2010nine of 112 patients were infected with wild\u2010type strains of <jats:italic>H.\u2003pylori<\/jats:italic>. Thirty\u2010nine patients were infected with strains with A2143G mutations. Twenty\u2010three patients were infected with both wild\u2010type strains and those with A2143G mutations. Only one patient was infected with the strain with A2142G mutation. <jats:italic>H.\u2003pylori<\/jats:italic> strains with A2143G or A2142G mutation had significantly higher MICs for clarithromycin.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The ASP\u2010PCR assay for 23S rRNA mutation of <jats:italic>H.\u2003pylori<\/jats:italic> is a useful method to detect clarithromycin\u2010resistant strains of <jats:italic>H.\u2003pylori<\/jats:italic> easily.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2006.04546.x","type":"journal-article","created":{"date-parts":[[2006,7,2]],"date-time":"2006-07-02T00:03:50Z","timestamp":1151798630000},"page":"1057-1063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Determination of mutations of the 23S rRNA gene of <i>Helicobacter\u2003pylori<\/i> by allele specific primer\u2010polymerase chain reaction method"],"prefix":"10.1111","volume":"22","author":[{"given":"Akiko","family":"Nakamura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahisa","family":"Furuta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohito","family":"Shirai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsushige","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Kajimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Soya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Hishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a011683"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.41.1.8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa001999"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90929-4"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1001\/jama.1996.03530320046033","article-title":"Consensus conference. Medical treatment of peptic ulcer disease. Practice guidelines. Practice parameters committee of the American College of Gastroenterology","volume":"275","author":"Soll AH","year":"1996","journal-title":"JAMA"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-126-4-199702150-00004"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(02)01134-8"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199510123331508"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2003.00162.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529609094541"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70119-8"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1067\/mcp.2001.113959"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2002.01368.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1128\/JCM.38.1.210-214.2000","article-title":"Detection of clarithromycin\u2010resistant Helicobacter pylori strains by a preferential homoduplex formation assay","volume":"38","author":"Maeda S","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2003.01406.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-8-705"},{"key":"e_1_2_6_18_2","first-page":"53","article-title":"[Detection of resistance to clarithromycin in clinical isolates of Helicobacter pylori from children and adults]","volume":"16","author":"Vega AE","year":"2003","journal-title":"Rev. Esp. Quimioter."},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.022111"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2005.00312.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2625"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1156-1157.2002"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.712"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3765-3769.2002"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.12.2621"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.11.038"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01962615"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.1.192-200.1992"},{"key":"e_1_2_6_29_2","first-page":"386","article-title":"Primary and acquired Helicobacter pylori resistance to clarithromycin, metronidazole, and amoxicillin \u2010 influence on treatment outcome","volume":"93","author":"Adamek RJ","year":"1998","journal-title":"Am. J. Gastroenterol."},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-3565(03)00288-X"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1358\/mf.2003.25.2.723687"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1517\/phgs.5.2.181.27483"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07707.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.37.1.39"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.1999.99299.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1128\/AAC.41.11.2550","article-title":"Macrolide resistance in Helicobacter pylori: mechanism and stability in strains from clarithromycin\u2010treated patients","volume":"41","author":"Hulten K","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13172.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2000.02072.x"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200107000-00005"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1746.2006.04546.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1746.2006.04546.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T20:30:33Z","timestamp":1697401833000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1746.2006.04546.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,5]]},"references-count":38,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1111\/j.1440-1746.2006.04546.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1746.2006.04546.x","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,5]]},"assertion":[{"value":"2006-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}