{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T06:25:21Z","timestamp":1779258321112,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,8,10]],"date-time":"2006-08-10T00:00:00Z","timestamp":1155168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Non\u2010invasive staging of human liver fibrosis is a desirable objective that remains under extensive evaluation. Animal model systems are often used for studying human liver disease and screening antifibrotic compounds. The aim of the present study was to investigate the potential use of serum N\u2010glycan profiles to evaluate liver fibrosis in a rat model.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Liver fibrosis and cirrhosis were induced in rats by oral administration of CCl<jats:sub>4<\/jats:sub>. Liver injury was assessed biochemically (alanine aminotransferase [ALT] activity, aspartate aminotransferase [AST] activity and total bilirubin) and histologically. The N\u2010glycan profile (GlycoTest) was performed using DNA sequencer\u2010assisted\u2013fluorophore\u2010assisted carbohydrate electrophoresis technology. In parallel, the effect of cotreatment with antifibrotic interferon\u2010\u03b3 (IFN\u2010\u03b3) was studied.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The biopsy scoring system showed that CCl<jats:sub>4<\/jats:sub> induced early fibrosis (<jats:italic>F<\/jats:italic>\u2003&lt;\u20031\u20132) in rats after 3\u2003weeks of treatment, and cirrhosis (F4) after 12\u2003weeks. Significant increases in ALT activity, AST activity and total bilirubin levels were detected only after 12\u2003weeks of CCl<jats:sub>4<\/jats:sub> treatment. GlycoTest showed three glycans were significantly altered in the CCl<jats:sub>4<\/jats:sub>\u2010goup. Peak 3 started at week 6, at an early stage in fibrosis development (<jats:italic>F<\/jats:italic>\u2003&lt;\u20031\u20132), whereas peaks 4 and 5 occurred at week 9, at which time mild liver fibrosis (<jats:italic>F<\/jats:italic>\u2003= 1\u20132) had developed. The changes in the CCl<jats:sub>4<\/jats:sub>\u2010IFN\u2010\u03b3 group were intermediate between the CCl<jats:sub>4<\/jats:sub>\u2010 and the control groups.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The GlycoTest is much more sensitive than biochemical tests for evaluating liver fibrosis\/cirrhosis in the rat model. The test can also be used as a non\u2010invasive marker for screening and monitoring the antifibrotic activity of potential therapeutic compounds.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2006.04553.x","type":"journal-article","created":{"date-parts":[[2006,8,10]],"date-time":"2006-08-10T09:30:59Z","timestamp":1155202259000},"page":"1148-1154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rating of CCl<sub>4<\/sub>\u2010induced rat liver fibrosis by blood serum glycomics"],"prefix":"10.1111","volume":"22","author":[{"given":"Liesbeth","family":"Desmyter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye\u2010Dong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marleen","family":"Praet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Jaworski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter","family":"Vervecken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"De Hemptinne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Contreras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.03.007"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00429-4"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.1999.tb00064.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2000.19347"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300437"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2003.12.008"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-002-4060-6"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2004.01.003"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(03)00010-2"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.035899"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.05.007"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9352863"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(03)00171-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20099"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20736"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.02.025"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.40881.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.11.018"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.5.1.15"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm.2004.42.6.681"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1006"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mop.0000133636.56790.4a"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1751"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(01)01292-5"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(99)00057-5"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwg041"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwg040"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2003.11.003"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/11.4.275"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840200104"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510230538"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(98)80322-X"},{"key":"e_1_2_6_34_2","first-page":"43","article-title":"Biological importance of glycosylation","volume":"96","author":"Dwek RA","year":"1998","journal-title":"Dev. 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