{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:20:06Z","timestamp":1761708006128},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,1,29]],"date-time":"2007-01-29T00:00:00Z","timestamp":1170028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aim:\u2002<\/jats:bold> The authors' previous report revealed that endothelin\u20101 might be released from B lymphocytes in cirrhotic patients with hypersplenism. Other investigators have shown that persistent exposure to environmental contaminants including arsenic might induce idiopathic portal hypertension. The aim of this study was to experimentally identify how endothelin\u20101 is involved in the development of idiopathic portal hypertension and which cells produce endothelin\u20101 in the spleen.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Portal pressure and venous endothelin\u20101 concentrations were measured in rats that were given sodium arsenate orally for long periods, and endothelin\u20101 expression levels in the spleen were assessed by staining. In a second experiment, B and T lymphocytes and monocyte\u2010derived macrophages cultured from healthy human peripheral blood were stimulated with sodium arsenite, sodium arsenate, lipopolysaccharide and interferon\u2010\u03b3. Endothelin\u20101 concentrations in the supernatants were measured by ELISA.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Arsenic exposure gradually increased portal pressure and venous endothelin\u20101 levels in rats. Endothelin\u20101 concentration in the supernatant did not change in every cell type stimulated with arsenic, but it increased in B lymphocytes and monocyte\u2010derived macrophages treated with lipopolysaccharide and interferon\u2010\u03b3.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The <jats:italic>in vivo<\/jats:italic> study indicated that arsenic might elevate portal pressure through mechanisms involving endothelin\u20101. In the <jats:italic>in vitro<\/jats:italic> study, lipopolysaccharide and interferon\u2010\u03b3 clearly induced endothelin\u20101 synthesis not only in monocyte\u2010derived macrophages but also in B lymphocytes, although arsenic treatment did not affect those cells. This study partially supports the hypothesis that idiopathic portal hypertension might be promoted by endothelin\u20101 overproduction from splenic B lymphocytes in response to certain substances. \u00a9 2007 The Authors; Journal compilation \u00a9 2007 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd<\/jats:p>","DOI":"10.1111\/j.1440-1746.2006.04822.x","type":"journal-article","created":{"date-parts":[[2007,1,29]],"date-time":"2007-01-29T14:41:12Z","timestamp":1170081672000},"page":"1134-1140","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Experimental investigation of the role of endothelin\u20101 in idiopathic portal hypertension"],"prefix":"10.1111","volume":"22","author":[{"given":"Emi","family":"Yamaguchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Yamanoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naofumi","family":"Nagasue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,29]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1645","article-title":"Idiopathic portal hypertension associated with polyclonal hyperimmunoglobulinemia. 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