{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:02:51Z","timestamp":1791158571789,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,11,18]],"date-time":"2009-11-18T00:00:00Z","timestamp":1258502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aim:\u2002<\/jats:bold> Liver fibrosis is closely associated with the progression of various chronic liver diseases. Fucoidan exhibits different biological properties such as anti\u2010inflammatory, anti\u2010oxidant and anti\u2010fibrotic activities. The aim of this study was to determine whether oral fucoidan administration inhibits N\u2010nitrosodiethylamine (DEN)\u2010induced liver fibrosis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Liver fibrosis was induced in rats by injecting DEN (50\u2003mg\/kg). Rats were given 2% of crude fucoidan solution or 2% of high\u2010molecular\u2010weight (HMW) fucoidan solution. They were divided into a crude fucoidan group, an HMW fucoidan group, a DEN alone group, a DEN\u2003+\u2003crude fucoidan group, a DEN\u2003+\u2003HMW fucoidan group and a control group.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Liver fibrosis and hepatic hydroxyproline levels were significantly more decreased in the DEN\u2003+\u2003HMW fucoidan group than in the DEN\u2010alone group. Anti\u2010fibrogenesis was unremarkable in the DEN\u2003+\u2003crude fucoidan group. Hepatic messenger RNA levels and immunohistochemistry of transforming growth factor beta 1 were markedly increased by DEN. This increase was attenuated by HMW fucoidan. Hepatic chemokine ligand 12 expression was increased by DEN. This increase was suppressed by HMW fucoidan. HMW fucoidan significantly decreased the DEN\u2010induced malondialdehyde levels. Also, fucoidan markedly increased metallothionein expression in the liver. Fucoidan was clearly observed in the liver by immunohistochemical staining in HMW fucoidan\u2010treated rats, while it was faintly stained in the livers of crude fucoidan\u2010treated rats.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These findings suggest that the HMW fucoidan treatment causes anti\u2010fibrogenesis in DEN\u2010induced liver cirrhosis through the downregulation of transforming growth factor beta 1 and chemokine ligand 12 expressions, and that scavenging lipid peroxidation is well\u2010incorporated in the liver.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2009.06187.x","type":"journal-article","created":{"date-parts":[[2009,11,18]],"date-time":"2009-11-18T08:30:25Z","timestamp":1258533025000},"page":"1692-1701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Attenuation of N\u2010nitrosodiethylamine\u2010induced liver fibrosis by high\u2010molecular\u2010weight fucoidan derived from <i>Cladosiphon okamuranus<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"Kyoumi","family":"Nakazato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Takada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Iha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeaki","family":"Nagamine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00429-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.327.7407.143"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra035021"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2006.tb00292.x"},{"key":"e_1_2_6_7_2","first-page":"313","article-title":"Sequential assessment of the intrahepatic expression of epidermal growth factor and transforming growth factor\u2010\u03b21 in hepatofibrogenesis of rat cirrhosis model","volume":"19","author":"Kurihara S","year":"2007","journal-title":"Int. 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