{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:46:18Z","timestamp":1789015578044,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T00:00:00Z","timestamp":1353369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Development of hepatic cirrhosis involves oxidative stress, inflammation, hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>)s activation and fibrosis. On the other hand, quercetin, a natural flavonoid is a potent antioxidant and activator of superoxide dismutase and catalase. The aim was to determinate the effect of quercetin on<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s and development of hepatic fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wistar male rats were chronically intoxicated with<jats:styled-content style=\"fixed-case\">CCl<\/jats:styled-content><jats:sub>4<\/jats:sub>for 8 weeks and concomitantly treated with 100\u2009mg\/kg per day of quercetin. Oxidative state, inflammation and fibrosis were evaluated. Effect of quercetin on apoptosis of<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>was determined by terminal deoxynucleotidyl transferase\u2010mediated dUTP nick end labeling reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty percent of reduction in fibrosis index was observed with quercetin treatment compared with control animals. Considerable reduction on hepatic enzymes was detected in the quercetin group. Expression of pro\u2010fibrotic genes (transforming growth factor\u2010\u03b2 [<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2], Collagen 1\u03b1 [<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ol\u20101\u03b1] and connective tissue growth factor [<jats:styled-content style=\"fixed-case\">CTGF<\/jats:styled-content>]) were decreased by quercetin. Quercetin increased gene expression and functional activity of antioxidant enzymes superoxide dismutase and catalase. Inflammatory index was highly reduced as determined by<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>staining and pro\u2010inflammatory cytokines expression and nuclear factor\u2010\u03baB activation were also inhibited. A significant reduction of 65% on activated<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>number was detected when rats were treated with quercetin. Quercetin also induced activation of matrix metalloproteinases<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>2 and<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>9 contributing to decreased index of fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Treatment with quercetin reduces oxidation and inflammation and also prevents liver fibrosis, through induction of<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>apoptosis and activation of<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1440-1746.2012.07262.x","type":"journal-article","created":{"date-parts":[[2012,9,18]],"date-time":"2012-09-18T13:04:17Z","timestamp":1347973457000},"page":"1865-1872","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Quercetin improves hepatic fibrosis reducing hepatic stellate cells and regulating pro\u2010fibrogenic\/anti\u2010fibrogenic molecules balance"],"prefix":"10.1111","volume":"27","author":[{"given":"Luis Daniel","family":"Hern\u00e1ndez\u2010Ortega","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca Estela","family":"Alc\u00e1ntar\u2010D\u00edaz","sequence":"additional","affiliation":[{"name":"Autonomous University of Nayarit Culture City \"Amado Nervo\" Tepic Nayarit M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Alberto","family":"Ruiz\u2010Corro","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Sandoval\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Bueno\u2010Topete","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Armendariz\u2010Borunda","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Mar\u00eda","family":"Salazar\u2010Montes","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, CUCS University of Guadalajara Guadalajara Jalisco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.CRJ04003X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60983-7"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/81.1.215S"},{"key":"e_1_2_5_5_1","first-page":"63","article-title":"Potential health effects of the dietary flavonol quercetin","volume":"50","author":"Hertog MGL","year":"1996","journal-title":"Eur. 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