{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T16:35:42Z","timestamp":1793205342110,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"s2","license":[{"start":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T00:00:00Z","timestamp":1126483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p><jats:bold>SUMMARY:\u2003<\/jats:bold> In the last 10\u2003years, many studies have focused on the non\u2010classical action of aldosterone. One of the most important new aspects of aldosterone is its pathogenic role as proinflammatory and profibrotic molecules. It has been reported that aldosterone induces myocardial fibrosis and vascular inflammation through up\u2010regulation of various proinflammatory and profibrotic cytokines. We investigated the effect of aldosterone and spironolactone, which is a non\u2010selective mineralocorticoid receptor antagonist, on monocyte chemoattractant peptide (MCP\u20101) and collagen synthesis in cultured mesangial and tubular epithelial cells. In addition, to evaluate the effect of spironolactone on diabetic nephropathy, we used Otsuka Long\u2010Evans Tokushima Fatty (OLETF) rats which are known type\u20032 diabetic animal models. Spironolactone treatment did not induce any significant change in blood glucose levels and blood pressure. However, spironolactone therapy significantly inhibited urinary albumin and MCP\u20101 excretion. Spironolactone treatment also suppressed renal mRNA expression for MCP\u20101, macrophage migration inhibitory factor (MIF) as well as intrarenal protein synthesis for ED\u20101 and MIF. Morphologically, spironolactone treatment significantly prevented glomerulosclerosis, collagen deposition and connective tissue growth factor (CTGF) expression in diabetic rats. In cultured cell experiments, aldosterone directly increased the MCP\u20101, collagen secretion and spironolactone treatment abolished aldosterone\u2010induced MCP\u20101 and collagen synthesis. Surprisingly, aldosterone treatment did not induce any significant change in TGF\u03b21 gene transcription. Finally, we found that NF\u2010kB activity was increased after stimulation with aldosterone and spironolactone therapy inhibited their activation. In addition, prior treatment with pyrrolidine dithiocarbamate (PDTC), which is a NF\u2010KB inhibitor, inhibited aldosterone\u2010induced MCP\u20101 protein secretion. These results suggest that aldosterone blockade could play a role in preventing the progression of diabetic nephropathy via anti\u2010inflammatory and antifibrotic mechanisms.<\/jats:p>","DOI":"10.1111\/j.1440-1797.2005.00455.x","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T08:51:36Z","timestamp":1126515096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Role of aldosterone in diabetic nephropathy"],"prefix":"10.1111","volume":"10","author":[{"given":"DAE RYONG","family":"CHA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YOUNG SUN","family":"KANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SANG YOUB","family":"HAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YI HWA","family":"JEE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KUM HYUN","family":"HAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HYOUNG KYU","family":"KIM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JEE YOUNG","family":"HAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YOUNG SIK","family":"KIM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,9,12]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.26.1.60"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199407283310408"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.71.3.503"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000140445.82949.54"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00707.x"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00929.x"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00913.x"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704131"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2004.04.020"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000044937.95080.E9"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01096.2001"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64454-9"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00311.x"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/17.6.985"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030490"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/147323000102900103"},{"key":"e_1_2_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(01)01237-2"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-004-1542-0"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjhyper.2004.06.029"},{"key":"e_1_2_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.02.005"},{"key":"e_1_2_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.1998.00820.x"}],"container-title":["Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1797.2005.00455.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1797.2005.00455.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T04:34:40Z","timestamp":1695702880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1797.2005.00455.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,12]]},"references-count":21,"journal-issue":{"issue":"s2","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1111\/j.1440-1797.2005.00455.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1797.2005.00455.x","archive":["Portico"],"relation":{},"ISSN":["1320-5358","1440-1797"],"issn-type":[{"value":"1320-5358","type":"print"},{"value":"1440-1797","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,12]]},"assertion":[{"value":"2005-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}