{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:13:54Z","timestamp":1770981234262,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T00:00:00Z","timestamp":1311811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT:<\/jats:title><jats:p><jats:bold>Aim:\u2003<\/jats:bold> To investigate the effects of recombinant human endostatin (Endostar) on peritoneum angiogenesis in a model of dialysate exposure in rats.<\/jats:p><jats:p><jats:bold>Methods:\u2003<\/jats:bold> Forty male Sprague\u2013Dawley rats were randomized to five groups: normal (group 1); uraemia (group 2); 4.25% peritoneal dialysate (PD) uraemic (group 3); uraemia + PD + recombinant human endostatin 10\u2003mg\/kg PD (group 4); and uraemia + PD + recombinant human endostatin 40\u2003mg\/kg PD (group 5). The uraemic rats model was established by 5\/6 nephrectomy. Endostatin was administrated by s.c. injection every other day, over 28\u2003days. After 28\u2003days of PD fluid exposure, immunohistochemistry and reverse transcript polymerase chain reaction were used to detect protein and mRNA expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in each group. Microvessel density (MVD) was measured by immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> Compared with group 1, the mRNA and protein expressions of VEGF and bFGF were significantly upregulated in groups 2 and 3 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Compared with group 3, the mRNA and protein expressions of VEGF and bFGF were significantly downregulated in groups 4 and 5 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Compared with group 4, the mRNA and protein expressions of VEGF and bFGF were significantly downregulated in group 5 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Compared with group 1, MVD was significantly upregulated in groups 2 and 3 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Compared with group 3, MVD was significantly downregulated in groups 4 and 5 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusion:\u2003<\/jats:bold> Endostar can effectively inhibit rat peritoneum neoangiogenesis and the effect was dose\u2010dependent.<\/jats:p>","DOI":"10.1111\/j.1440-1797.2011.01465.x","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T11:11:10Z","timestamp":1301656270000},"page":"599-606","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of recombinant human endostatin on peritoneal angiogenesis in peritoneal dialysis rats"],"prefix":"10.1111","volume":"16","author":[{"given":"ZHAN\u2010ZHENG","family":"ZHAO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YING","family":"CAO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZHANG\u2010SUO","family":"LIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JING","family":"XIAO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LIN","family":"TANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PEI","family":"WANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XIAN\u2010HUI","family":"LIANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,28]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1681\/ASN.V133721","article-title":"Antiangiogenic and antifibroticgene therapy in a chronic infusion model of peritoneal dialysis in rats","volume":"13","author":"Margetts PJ","year":"2002","journal-title":"J. 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