{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:58:40Z","timestamp":1792148320284,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T00:00:00Z","timestamp":1229040000000},"content-version":"vor","delay-in-days":4577,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[1996,6]]},"abstract":"<jats:p>Twenty Intramucosal tumors of \u2018carclnoma\u2010ln\u2010adenoma\u2019 and 43 adenomas (39 pylorlc gland type, 4 Intestinal type) of the gall\u2010bladder were studied to establish more precise hlsto\u2010loglcal criteria of carcinoma or adenoma in cases of \u2018carcinoma in pylorlc gland type adenoma\u2019, to compare carcinoma in adenoma with pure, that is, without adenomatous components, carcinoma, and to confirm the benign nature of spindle cell foci in the adenomas. Ki\u201067 and p53 immunostaining and nuclear morphometry were used. Eight pure intramucosal cancers were used as controls. The formalin\u2010fixed, paraffin\u2010embedded sections were stained with p53 and Ki\u201067 antibodies. Spindle cell foci were observed only in the adenoma area of the pylorlc gland type, with a frequency of 23% in 39 adenomas, and of 45% in 20 tumors of carclnoma\u2010Jn\u2010adenoma. Ki\u201067 staining was negative in 129 of 130 spin\u2010die cell foci examined, regardless of their size, and positive in only one focus (550 \u03bcm in size, Ki\u201067 index 0.2%). All of the spindle cell foci were negative for p53 stain. The Ki\u201067 positive index was 36.6 \u00b15.6% in the 8 pure carcinomas, and 12.5 \u00b11.9% in the carcinoma area of 16 tumors with carci\u2010noma\u2010in\u2010adenoma, while it was 7.9\u00b1 1.7% and in the adenoma areas of 16 tumors with carcinoma\u2010in\u2010adenoma and 4.9 \u00b1 0.5% in the 32 pure pyloric gland adenomas. The p53\u2010protein overexpression was found in seven of eight pure intramucosal cancers, and in one of 16 cancer components of carcinoma\u2010in\u2010adenoma. However, it was not found in any of 16 adenoma components of carcinoma\u2010in\u2010adenoma, and 35 adenomas. Cells of the cancer tissue of carcinoma\u2010in\u2010adenoma showed a significantly larger nuclear area and a larger nuclear minor axis than those of the pyloric gland type adenomas, as well as other architectural and cytologic abnormalities differing from the features of adenomas. These results suggest that clustered spindle cells do not indicate a malignant transformation of adenoma cells and that carcinomas in carcinoma\u2010in\u2010adenoma are different from pyloric gland type adenomas in terms of morphology and proliferative activity. Moreover, the results of the present study indicate that carcinomas in carcinoma\u2010in\u2010adenoma are lower in malignancy than pure carcinomas, and that their genetic abnormality may differ from that of pure carcinomas.<\/jats:p>","DOI":"10.1111\/j.1440-1827.1996.tb03633.x","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T15:08:39Z","timestamp":1229094519000},"page":"426-435","source":"Crossref","is-referenced-by-count":10,"title":["p53 and Ki\u201067 immunoreactivity and nuclear morphometry of \u2018carcinoma\u2010in\u2010adenoma\u2019 and adenoma of the gall\u2010bladder"],"prefix":"10.1111","volume":"46","author":[{"given":"Kazuo","family":"Takei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenobu","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Itoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,12]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"International Histological Classification of Tumors","author":"Albores\u2010Saavedra J","year":"1991"},{"key":"e_1_2_1_3_2","first-page":"145","article-title":"Non\u2010neoplastic polypoid lesions and adenomas of the gall\u2010bladder","volume":"1","author":"Albores\u2010Saavedra J","year":"1993","journal-title":"Pathol. 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