{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:54:25Z","timestamp":1697072065926},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T00:00:00Z","timestamp":1076976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:p>Topoisomerase\u2010II alpha (Topo\u2010II alpha) is known as a cell cycle\u2010related intranuclear marker. To the best of our knowledge, the expression of Topo\u2010II alpha on extranuclear sites has not been reported. The aim of the present study was to determine the usefulness of Topo\u2010II alpha immunostaining for detecting the lipoblasts that are essential to diagnosing liposarcoma. Surgical specimens, including benign lipomatous tumors (four cases), well\u2010differentiated liposarcomas (three cases), myxoid liposarcomas (six cases), pleomorphic liposarcomas (two cases), dedifferentiated liposarcomas (two cases), myxoid malignant fibrous histiocytomas (six cases), and one case of mesenteric panniculitis, were studied. Samples were immunostained using antibodies for Topo\u2010II alpha, S\u2010100 protein and Ki\u201067. In addition, we used the western blot method to investigate immunohistochemical\u2010affinity in adipocytes. Mature adipocytes and lipoblasts in all of the benign and malignant lipomatous tumors intensively expressed cell contours positivity for Topo\u2010II alpha. Cytoplasm of the lipoblasts occasionally reacted to the antibody and highlighted intracytoplasmic small unilocular, multivacuolated, or bubble\u2010like patterns. Western blot analysis confirmed a 70\u2003kDa product reactive to Topo\u2010II alpha in the cell membrane fragment of mature adipocytes. S\u2010100 protein expressed adipocytes and lipoblasts, but the detection of lipoblasts was not as easy as in Topo\u2010II alpha immunostaining. Immunoreactivity of Ki\u201067 was limited to the nuclei, and the nuclear labeling index of Ki\u201067 correlated with that of Topo\u2010II alpha. The immunoreactivity of Topo\u2010II alpha for lipoblasts was more sensitive and obvious than those of S\u2010100 protein. Immunostaining using the antibody for Topo\u2010II alpha seems to be useful in recognizing lipoblasts that have been overlooked in hematoxylin\u2013eosin\u2010stained preparations, and is a useful marker for diagnosing liposarcoma.<\/jats:p>","DOI":"10.1111\/j.1440-1827.2003.01600.x","type":"journal-article","created":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T10:19:26Z","timestamp":1077013166000},"page":"145-150","source":"Crossref","is-referenced-by-count":7,"title":["Unique cell membrane expression of topoisomerase\u2010II alpha as a useful diagnostic marker of liposarcoma"],"prefix":"10.1111","volume":"54","author":[{"given":"Hideko","family":"Endo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuyoshi","family":"Hirokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naozumi","family":"Ishimaru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Sakaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Sano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/103.1.20"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200205000-00006"},{"key":"e_1_2_5_4_2","first-page":"901","article-title":"DNA topoisomerase II alpha and Ki\u201067 in human adrenocortical neoplasms: a possible marker of differentiation between adenomas and carcinomas","volume":"10","author":"Iino K","year":"1997","journal-title":"Mod Pathol"},{"key":"e_1_2_5_5_2","first-page":"186","article-title":"Immunohistochemical and two\u2010parameter flow cytometric studies of DNA topoisomerase II alpha in human epithelial ovarian carcinoma and germ cell tumor","volume":"11","author":"Tanoguchi K","year":"1998","journal-title":"Mod Pathol"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.10.6031"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.14.7859"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.1995.tb03475.x"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200004000-00013"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2002.01330.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00694921"},{"key":"e_1_2_5_12_2","first-page":"95","article-title":"S\u2010100 protein staining in liposarcoma. Its diagnostic utility in the high\u2010grade myxoid (round cell) variant","volume":"4","author":"Angelo PDT","year":"1996","journal-title":"Appl Immunohisto"},{"key":"e_1_2_5_13_2","first-page":"997","article-title":"Immunohistochemical study of DNA topoisomerase II in human gastric disorders","volume":"149","author":"Yabuki N","year":"1996","journal-title":"Am J Pathol"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1827.2003.01600.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1827.2003.01600.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T17:22:07Z","timestamp":1696872127000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1827.2003.01600.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,17]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["10.1111\/j.1440-1827.2003.01600.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1827.2003.01600.x","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,17]]}}}