{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:19:22Z","timestamp":1789096762967,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,8,30]],"date-time":"2007-08-30T00:00:00Z","timestamp":1188432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>Wilms' tumor 1 (WT1) is a useful marker for the diagnosis of acute leukemia and myelodysplastic syndromes (MDS). In the current study quantitative reverse transcription\u2013polymerase chain reaction and immunostaining were used simultaneously to examine the relationship between <jats:italic>WT1<\/jats:italic> RNA and protein level and also to evaluate WT1 as a tool to differentiate aplastic anemia (AA) and MDS refractory anemia (RA). Three types of <jats:italic>WT1<\/jats:italic> messages (total, exon 5(+) and KTS(+)) and WT1 immunostaining of these diseases were analyzed. An increase of all three <jats:italic>WT1<\/jats:italic> messages in high\u2010grade MDS and acute leukemia was observed as compared with the normal control, whereas there was no significant difference in <jats:italic>WT1<\/jats:italic> message between AA and RA, suggesting that <jats:italic>WT1<\/jats:italic> message is not a good tool to discriminate AA and RA. No significant difference was observed between normal and RA, except for exon 5 message. Three <jats:italic>WT1<\/jats:italic> message levels had a significant correlation, suggesting that the total WT1 message is sufficient for clinical practice. Positive immunostaining of WT1 was observed only in the portion of acute leukemia and overt leukemia (OL) transformed from MDS with a high <jats:italic>WT1<\/jats:italic> message level, suggesting the relatively high detection threshold of WT1 protein with the immunostaining method.<\/jats:p>","DOI":"10.1111\/j.1440-1827.2007.02153.x","type":"journal-article","created":{"date-parts":[[2007,9,3]],"date-time":"2007-09-03T04:00:58Z","timestamp":1188792058000},"page":"645-651","source":"Crossref","is-referenced-by-count":8,"title":["Wilms' tumor 1 message and protein expression in bone marrow failure syndrome and acute 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