{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T02:30:44Z","timestamp":1697250644794},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,31]],"date-time":"2005-03-31T00:00:00Z","timestamp":1112227200000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:p><jats:bold>Objective:\u2003<\/jats:bold> The aim of this study was to investigate the number of Clara cells and the production and secretion of Clara cell 16\u2003kDa protein (CC16) in a murine model of allergen\u2010induced airway inflammation, as well as the effects of N\u2010acetylcysteine (NAC) on CC16 and Clara cell numbers, in order to determine the mechanism of the anti\u2010inflammatory effect of NAC.<\/jats:p><jats:p><jats:bold>Methodology:\u2003<\/jats:bold> BALB\/c mice were divided into control, ovalbumin (OVA) and NAC groups. An allergen\u2010induced airway inflammation model (OVA group) was established by sensitizing and challenging mice with OVA. NAC was administered as an oral treatment. The number of Clara cells and the production of CC16 were determined by immunohistochemistry. The CC16 levels in bronchoalveolar lavage fluid (BALF) were determined by Western blotting.<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> The proportion of Clara cells in terminal and respiratory bronchioles significantly decreased in the OVA group compared to the control group (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). NAC treatment did not change the proportion of Clara cells in the OVA group (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05). CC16 production by Clara cells in the OVA groups was significantly lower than that of the control group (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01), but was elevated following NAC treatment (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). The CC16 level in BALF of the OVA group was lower than that of the control group (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01), but was elevated by NAC treatment (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). NAC reduced the total number of white cells and the percentage of eosinophils in BALF. Moreover, it inhibited airway inflammation.<\/jats:p><jats:p><jats:bold>Conclusions:\u2003<\/jats:bold> The number of Clara cells and the production and secretion of CC16 were reduced in a murine model of allergen\u2010induced airway inflammation. Antioxidants can enhance the expression of CC16, which might be a mechanism by which they suppress airway inflammation.<\/jats:p>","DOI":"10.1111\/j.1440-1843.2005.00698.x","type":"journal-article","created":{"date-parts":[[2005,3,31]],"date-time":"2005-03-31T11:04:49Z","timestamp":1112267089000},"page":"157-163","source":"Crossref","is-referenced-by-count":2,"title":["The effect of N\u2010acetylcysteine on Clara cells and Clara cell 16\u2003kDa protein in a murine model of allergen\u2010induced airway inflammation"],"prefix":"10.1111","volume":"10","author":[{"given":"Xiaomeng","family":"NIE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"LI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"CAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimin","family":"DAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)91006-B"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(93)90077-H"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(86)90135-9"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000738"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.161.5.9812157"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.2206012"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/pupt.2001.0319"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.3.4357"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236807"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.2001.0917"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780352"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-140407-9.50013-1"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1183\/09031936.98.11030726","article-title":"Quantification of Clara cell protein in rat and mouse biological fluids using a sensitive immunoassay","volume":"11","author":"Halatek T","year":"1998","journal-title":"Eur. Respir. J."},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050015"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91855-8"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901213400306"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/135245859600100621"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.17.2.2676"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.5.L864"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00186.2002"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.2.4718"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.16.5.9160833"},{"key":"e_1_2_6_24_2","first-page":"77","volume-title":"Drugs in Bronchial Mucology","author":"Ventresca GP","year":"1989"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(89)90066-X"},{"key":"e_1_2_6_26_2","first-page":"205","volume-title":"Antioxidants in Disease: Mechanisms and Therapy (Advances in Pharmacology","author":"Cotgreave IA","year":"1997"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007203430304"},{"key":"e_1_2_6_28_2","first-page":"240","article-title":"Reactive oxygen species in asthma","volume":"10","author":"Barnes PJ","year":"2000","journal-title":"Eur. Respir. Rev."},{"key":"e_1_2_6_29_2","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1096\/fasebj.13.13.1845","article-title":"Supplementation of N\u2010acetylcysteine inhibits NF\u2010\u03baB activation and protects against alloxan\u2010induced diabetes in CD\u20101 mice","volume":"13","author":"Ho E","year":"1999","journal-title":"FASEB J."},{"key":"e_1_2_6_30_2","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.4049\/jimmunol.157.4.1630","article-title":"In vivo antioxidant treatment suppresses nuclear factor\u2010\u03baB activation and neutrophilic lung inflammation","volume":"157","author":"Blackwell TS","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701242"}],"container-title":["Respirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1843.2005.00698.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1843.2005.00698.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T05:33:36Z","timestamp":1696916016000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1843.2005.00698.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1111\/j.1440-1843.2005.00698.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1843.2005.00698.x","archive":["Portico"],"relation":{},"ISSN":["1323-7799","1440-1843"],"issn-type":[{"value":"1323-7799","type":"print"},{"value":"1440-1843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}