{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T14:05:00Z","timestamp":1773669900983,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,31]],"date-time":"2007-10-31T00:00:00Z","timestamp":1193788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p><jats:bold>Background and objectives:\u2003<\/jats:bold> The pathogenesis and the optimal treatment of eosinophilic pleural effusions are unknown. We aimed to examine whether pneumothorax\u2010associated pleural eosinophilia in mice is dependent on tumour necrosis factor (TNF)\u2010alpha, and whether it is affected by systemic administration of corticosteroids.<\/jats:p><jats:p><jats:bold>Methods:\u2003<\/jats:bold> Mice were injected intrapleurally with 0.4\u2003mL air to create pneumothoraces. Animals were sacrificed 24 or 48\u2003h later, and pleural lavage (PL) was performed. In the first experiment, comparisons were made between wild\u2010type and TNF\u2010\u03b1 knockout mice with pneumothorax. In the second experiment, wild\u2010type mice were injected intraperitoneally with different doses of dexamethasone (0, 0.25, 0.5 and 1\u2003mg\/kg), 5\u2003min before and 24\u2003h after the induction of pneumothorax.<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> After induction of a pneumothorax, TNF\u2010\u03b1 knockout mice had significantly fewer total number of cells (<jats:italic>P\u2003<\/jats:italic>=\u20030.004), mononuclear cells (<jats:italic>P\u2003<\/jats:italic>=\u20030.01), neutrophils (<jats:italic>P\u2003<\/jats:italic>=\u20030.017) and eosinophils (<jats:italic>P\u2003<\/jats:italic>=\u20030.002) in their PL compared with wild\u2010type animals. TNF\u2010\u03b1 was detected in the PL of most of the control mice but not in TNF\u2010\u03b1 knockouts. Dexamethasone induced a significant, dose\u2010dependent reduction of PL total cells (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.001), eosinophils (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.001), mononuclear cells (<jats:italic>P\u2003<\/jats:italic>=\u20030.007) and lymphocytes (<jats:italic>P\u2003<\/jats:italic>=\u20030.04) at 48\u2003h, and significantly reduced the number of PL total cells (<jats:italic>P\u2003<\/jats:italic>=\u20030.045) and eosinophils (<jats:italic>P\u2003<\/jats:italic>=\u20030.005) at 24\u2003h. Furthermore, dexamethasone prevented eosinophil infiltration of lung and pleural tissue.<\/jats:p><jats:p><jats:bold>Conclusion:\u2003<\/jats:bold> Pneumothorax\u2010associated pleural eosinophilia in mice is TNF\u2010\u03b1\u2010dependent and is significantly attenuated by corticosteroid treatment. In addition, both TNF\u2010\u03b1 deficiency and dexamethasone treatment were associated with a significant reduction of other types of inflammatory cells in PL.<\/jats:p>","DOI":"10.1111\/j.1440-1843.2007.01153.x","type":"journal-article","created":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T01:21:17Z","timestamp":1193880077000},"page":"73-78","source":"Crossref","is-referenced-by-count":12,"title":["Pneumothorax\u2010associated pleural eosinophilia is tumour necrosis factor\u2010alpha\u2010dependent and attenuated by steroids"],"prefix":"10.1111","volume":"13","author":[{"given":"Ioannis","family":"KALOMENIDIS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charalampos","family":"MOSCHOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Androniki","family":"KOLLINTZA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioanna","family":"SIGALA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgios T.","family":"STATHOPOULOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spyros A.","family":"PAPIRIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"LIGHT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charis","family":"ROUSSOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00063198-200307000-00002"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcp.0000127902.37822.13"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.4.2978"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-125"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-004-2519-y"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2003.00529.x"},{"key":"e_1_2_6_8_2","first-page":"63","volume-title":"Textbook of Pleural Disease.","author":"Lee YCG","year":"2003"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.98.2.348"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.4.1397"},{"key":"e_1_2_6_11_2","first-page":"41","article-title":"Pharmacological modulation of lipopolysaccharide\u2010induced pleural eosinophilia in the rat; a role for a newly generated protein","volume":"248","author":"Bozza PT","year":"1993","journal-title":"Eur. 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