{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:54:55Z","timestamp":1771005295914,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,5,21]],"date-time":"2008-05-21T00:00:00Z","timestamp":1211328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:p><jats:bold>Background and objective:\u2003<\/jats:bold> Expression of genes involved in DNA repair and\/or DNA synthesis, including ribonucleotide reductase M1 (RRM1) and excision repair cross\u2010complementation 1 (ERCC1) has been reported to be associated with chemosensitivity to platinum agents and gemcitabine. The aim of this study was to test whether similar associations would be seen between mRNA expression for the RRM1, ERCC1 and ERCC2 genes and <jats:italic>in vitro<\/jats:italic> chemosensitivity in lung cancer.<\/jats:p><jats:p><jats:bold>Methods:\u2003<\/jats:bold> Using a panel of 20 lung cancer cell lines, including 15 NSCLC and 5 small cell lung cancers (SCLC), the mRNA expression levels for the RRM1, ERCC1 and ERCC2 genes were examined by quantitative real\u2010time reverse transcription PCR. The <jats:italic>in vitro<\/jats:italic> cytotoxicity of cisplatin, carboplatin and gemcitabine was assessed using a tetrazolium\u2010based colorimetric assay (3\u2010[4,5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5\u2010diphenyltetrazoliumbromide (MTT) assay).<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> Significantly, higher RRM1 mRNA expression was found in SCLC compared with NSCLC. However, there were no correlations between mRNA expression of the ERCC1, ERCC2 and RRM1 genes and chemosensitivity to cisplatin, carboplatin or gemcitabine.<\/jats:p><jats:p><jats:bold>Conclusions:\u2003<\/jats:bold> These <jats:italic>in vitro<\/jats:italic> results suggest that further studies are needed to evaluate the expression of the RRM1, ERCC1 and ERCC2 genes as predictive biomarkers for sensitivity to platinum agents and gemcitabine.<\/jats:p>","DOI":"10.1111\/j.1440-1843.2008.01302.x","type":"journal-article","created":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T00:59:31Z","timestamp":1211417971000},"page":"510-517","source":"Crossref","is-referenced-by-count":22,"title":["mRNA expression of RRM1, ERCC1 and ERCC2 is not associated with chemosensitivity to cisplatin, carboplatin and gemcitabine in human lung cancer cell lines"],"prefix":"10.1111","volume":"13","author":[{"given":"Junichi","family":"SHIMIZU","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitsugu","family":"HORIO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotaka","family":"OSADA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyoaki","family":"HIDA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"HASEGAWA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaoru","family":"SHIMOKATA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"TAKAHASHI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitaka","family":"SEKIDO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"YATABE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11043"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.57.1.43"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)82038-3"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdl377"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa011954"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2007.05.017"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.06.1101"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060570"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa065411"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-7372(98)90056-1"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206419"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-7754(03)00281-1"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700148"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.1.4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdl300"},{"key":"e_1_2_6_17_2","first-page":"2286","article-title":"Low ERCC1 expression correlates with prolonged survival after cisplatin plus gemcitabine chemotherapy in non\u2010small cell lung cancer","volume":"8","author":"Lord RV","year":"2002","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2005.06.013"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.09.7915"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdm072"},{"key":"e_1_2_6_21_2","first-page":"3645","article-title":"HER\u20102\/Neu alterations in non\u2010small cell lung cancer: a comprehensive evaluation by real time reverse transcription\u2010PCR, fluorescence in situ hybridization, and immunohistochemistry","volume":"9","author":"Pellegrini C","year":"2003","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63093-3"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc070742"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3363"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01509.x"},{"key":"e_1_2_6_26_2","first-page":"4204","article-title":"Overexpression of ribonucleotide reductase as a mechanism of resistance to 2,2\u2010difluorodeoxycytidine in the human KB cancer cell line","volume":"59","author":"Goan YG","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0121"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0989"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.12.002"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-040006"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(00)00441-X"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/14.10.2177"},{"key":"e_1_2_6_33_2","first-page":"773","article-title":"Expression of ERCC1 antisense RNA abrogates gemcitabine\u2010mediated cytotoxic synergism with cisplatin in human colon tumor cells defective in mismatch repair but proficient in nucleotide excision repair","volume":"6","author":"Yang LY","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117388"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.1.309"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0079"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318155a637"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.127.3.978"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.08.067"},{"key":"e_1_2_6_40_2","first-page":"5457","article-title":"Regulation of cisplatin resistance and homologous recombinational repair by the TFIIH subunit XPD","volume":"62","author":"Aloyz R","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011104509759"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.09.130"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-174"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/73439"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.12.3968"}],"container-title":["Respirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1843.2008.01302.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1843.2008.01302.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T23:37:59Z","timestamp":1696894679000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1843.2008.01302.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,21]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1111\/j.1440-1843.2008.01302.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1843.2008.01302.x","archive":["Portico"],"relation":{},"ISSN":["1323-7799","1440-1843"],"issn-type":[{"value":"1323-7799","type":"print"},{"value":"1440-1843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,21]]}}}