{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T04:14:21Z","timestamp":1697343261200},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,8,18]],"date-time":"2011-08-18T00:00:00Z","timestamp":1313625600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Glycohemoglobins (GHb) are the products of irreversible non\u2010enzymatic reactions between glucose and the hemoglobin molecule. Glycosylation of proteins in general can modify protein structure and alter catalytic properties, thereby causing cellular dysfunction. The aim of the present study was to test the hypothesis that protein glycosylation levels in premature infants are elevated compared with full\u2010term infants (neonates). Blood GHb levels in pre\u2010term and full\u2010term infants were studied, and the <jats:italic>in vitro<\/jats:italic> glycosylation of erythrocytes obtained from both pre\u2010term and full\u2010term infants was assessed.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Cord\u2010blood from 31 pre\u2010term and 11 full\u2010term infants was collected and GHb levels were determined using affinity columns. Erythrocytes from the cord blood of 17 additional infants (eight pre\u2010term, \u226436 weeks gestation, and nine full\u2010term, \u226537 weeks gestation) were obtained and incubated for 24\u2003h in a high\u2010glucose medium. Baseline and post\u2010incubation GHb levels were calculated to determine the <jats:italic>in vitro<\/jats:italic> susceptibility of pre\u2010term versus full\u2010term infants to glycosylation.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Blood GHb levels were significantly higher (<jats:italic>P<\/jats:italic> &lt; 0.01) in full\u2010term compared with pre\u2010term infants. The percent increase in GHb formation <jats:italic>in vitro<\/jats:italic> was similar between the erythrocytes of full\u2010term and those of pre\u2010term infants.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Contrary to the original hypothesis, the erythrocytes of pre\u2010term infants do not show increased glycosylation of proteins when compared with those of full\u2010term infants.<\/jats:p>","DOI":"10.1111\/j.1442-200x.2011.03379.x","type":"journal-article","created":{"date-parts":[[2011,4,12]],"date-time":"2011-04-12T10:38:00Z","timestamp":1302604680000},"page":"480-482","source":"Crossref","is-referenced-by-count":0,"title":["Effect of prematurity on protein glycosylation in the newborn"],"prefix":"10.1111","volume":"53","author":[{"given":"Gerald B.","family":"Whitton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramasubbareddy","family":"Dhanireddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushil K.","family":"Jain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005721-200411000-00008"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.27.6.1259"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90104-0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022243"},{"key":"e_1_2_6_6_2","first-page":"170","article-title":"Comparison of hematologic parameters in normal streptozotocin\u2010induced diabetic rats","volume":"42","author":"Alder VA","year":"1992","journal-title":"Lab. 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