{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:46:28Z","timestamp":1780447588933,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T00:00:00Z","timestamp":1334102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p><jats:bold>Objectives:\u2003<\/jats:bold> To analyze the crystal components and matrix proteins of urinary stones by proteomic analysis using liquid chromatography\u2013tandem mass spectrometry.<\/jats:p><jats:p><jats:bold>Methods:\u2003<\/jats:bold> Urinary stones were obtained from patients with gout and hyperuricemia. The outside and inside of the stones were measured non\u2010destructively with a micro area X\u2010ray diffractometer. After stones were powdered, extracted proteins were analyzed by proteomic analysis.<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> Of 17 investigated stones, seven were composed of calcium oxalate monohydrate or calcium oxalate dihydrate, seven were of uric acid, and three were a mixture of calcium oxalate monohydrate and uric acid. In calcium oxalate monohydrate or calcium oxalate dihydrate stones, osteopontin, uromodulin, albumin, protein Z, prothrombin, protein S, hemoglobin and histone H4 were identified. In uric acid stones, uromodulin, albumin, hemoglobin, calgranulins and immunoglobin\u2003G fragments were detected. Mixed stones of calcium oxalate monohydrate and uric acid contained both Ca\u2010binding proteins and abundant proteins. Matrix proteins were different when the crystal components of the stone were different, even when from the same patient.<\/jats:p><jats:p><jats:bold>Conclusions:\u2003<\/jats:bold> Proteins, such as uromodulin and albumin, are often detected in stones, regardless of crystal components. However, osteopontin, prothrombin, protein S and protein Z are identified specifically in calcium oxalate stones. Furthermore, immunoglobin\u2003G fragments are detected in uric acid stones. The role of these specific proteins in the different types of stones can be of particular interest.<\/jats:p>","DOI":"10.1111\/j.1442-2042.2012.03005.x","type":"journal-article","created":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T23:51:16Z","timestamp":1334188276000},"page":"765-772","source":"Crossref","is-referenced-by-count":38,"title":["Comparison of matrix proteins in different types of urinary stone by proteomic analysis using liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1111","volume":"19","author":[{"given":"Kiyoko","family":"Kaneko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riei","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Izumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyo","family":"Yamanobe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,11]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(68)90209-X"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.09.034"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)35454-X"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.2741\/1347"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82453-X"},{"key":"e_1_2_8_7_2","first-page":"623","article-title":"Detection of osteopontin as matrix protein in calcium\u2010containing urinary stones","volume":"43","author":"Yamate T","year":"1997","journal-title":"Hinyokika Kiyo"},{"issue":"14","key":"e_1_2_8_8_2","first-page":"S408","article-title":"Gene expression of prothrombin in human and rat kidneys: basic and clinical approach","volume":"10","author":"Suzuki K","year":"1999","journal-title":"J. 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