{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:03:09Z","timestamp":1791266589585,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T00:00:00Z","timestamp":1340064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:bold>Objectives:\u2003<\/jats:bold> To study the altered expression of TWIK\u2010related arachidonic acid\u2010activated K<jats:sup>+<\/jats:sup> channel in the L6\u2013S1 spinal cord of rats after complete bladder outlet obstruction, and to investigate the role of TWIK\u2010related arachidonic acid\u2010activated K<jats:sup>+<\/jats:sup> channel in the neurogenic mechanism of bladder dysfunction.<\/jats:p><jats:p><jats:bold>Methods:\u2003<\/jats:bold> Female Sprague\u2013Dawley rats were randomly divided into a complete bladder outlet obstruction group and a sham\u2010operated control group. Cystometry was carried out and tissues of L6\u2013S1 spinal cord were obtained for detection of TWIK\u2010related arachidonic acid\u2010activated K<jats:sup>+<\/jats:sup> channel mRNA and protein by real\u2010time polymerase chain reaction, western blot and immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:\u2003<\/jats:bold> The bladder outlet obstruction rat model was established. Real\u2010time polymerase chain reaction, western blot and immunohistochemistry showed that the expression of TWIK\u2010related arachidonic acid\u2010activated K<jats:sup>+<\/jats:sup> channel was lower in the L6\u2013S1 spinal cord of the bladder outlet obstruction rats, compared with the control rats.<\/jats:p><jats:p><jats:bold>Conclusions:\u2003<\/jats:bold> Downregulation of TWIK\u2010related arachidonic acid\u2010activated K<jats:sup>+<\/jats:sup> channel might enhance the excitability of the neurons and increase the sensitivity of the bladder, probably providing a new study model of overactive bladder secondary to bladder outlet obstruction.<\/jats:p>","DOI":"10.1111\/j.1442-2042.2012.03072.x","type":"journal-article","created":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T08:42:11Z","timestamp":1340095331000},"page":"944-950","source":"Crossref","is-referenced-by-count":2,"title":["Downregulation of TWIK\u2010related arachidonic acid\u2010activated K<sup>+<\/sup> channel in the spinal cord of rats after complete bladder outlet obstruction"],"prefix":"10.1111","volume":"19","author":[{"given":"Xilian","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyou","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,19]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2004.04562.x"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06333.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-009-0210-6"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08471.x"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00300255"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00294337"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655999750042123"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)35836-6"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.12.3297"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200110000-00034"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.10.027"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.02.052"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66406-3"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2009.06.012"},{"key":"e_1_2_8_16_2","first-page":"256","article-title":"Considerations for the use of anesthetics in neurotoxicity studies","volume":"60","author":"Karmarkar SW","year":"2010","journal-title":"Comp. 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