{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:33:28Z","timestamp":1791117208215,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,8,3]],"date-time":"2011-08-03T00:00:00Z","timestamp":1312329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>A<jats:sc>bstract<\/jats:sc><\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Chemical burns cause depletion of limbal stem cells and eventually lead to corneal opacity and visual loss. We investigated the long\u2010term effectiveness of autologous cultured limbal stem cell grafts in patients with limbal stem cell deficiency.<\/jats:p><jats:p><jats:bold>Design:\u2002<\/jats:bold> Prospective, non\u2010comparative interventional case series.<\/jats:p><jats:p><jats:bold>Participants:\u2002<\/jats:bold> Sixteen eyes from 16 patients with severe, unilateral limbal stem cell deficiency caused by chemical burns.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Autologous <jats:italic>ex vivo<\/jats:italic> cultured limbal stem cells were grafted onto the recipient eye after superficial keratectomy.<\/jats:p><jats:p><jats:bold>Main Outcome Measures:\u2002<\/jats:bold> Clinical parameters of limbal stem cell deficiency (stability\/transparency of the corneal epithelium, superficial corneal vascularization and pain\/photophobia), visual acuity, cytokeratin expression on impression cytology specimens and histology on excised corneal buttons.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> At 12\u2003months post\u2010surgery, evaluation of the 16 patients showed that 10 (62.6%) experienced complete restoration of a stable and clear epithelium and 3 (18.7%) had partially successful outcomes (re\u2010appearance of conjunctiva in some sectors of the cornea and instable corneal surface). Graft failure (no change in corneal surface conditions) was seen in three (18.7%) patients. Penetrating keratoplasty was performed in seven patients, with visual acuity improving up to 0.8 (best result). For two patients, regeneration of the corneal epithelium was confirmed by molecular marker (p63, cytokeratin 3, 12 and 19, mucin 1) analysis. Follow\u2010up times ranged from 12 to 50\u2003months.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Grafts of autologous limbal stem cells cultured onto fibrin glue discs can successfully regenerate the corneal epithelium in patients with limbal stem cell deficiency, allowing to perform successful cornea transplantation and restore vision.<\/jats:p>","DOI":"10.1111\/j.1442-9071.2011.02609.x","type":"journal-article","created":{"date-parts":[[2011,6,13]],"date-time":"2011-06-13T18:57:49Z","timestamp":1307991469000},"page":"255-267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Long\u2010term effectiveness of autologous cultured limbal stem cell grafts in patients with limbal stem cell deficiency due to chemical 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