{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T14:11:16Z","timestamp":1697206276590},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,10]],"date-time":"2004-02-10T00:00:00Z","timestamp":1076371200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chinese Journal of Digestive Diseases"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p><jats:bold>OBJECTIVE:\u2003<\/jats:bold> To investigate in rats the role of endothelin (ET)\u20101 gene expression in the development and progression of acute gastric mucosal lesions (AGML) induced by stress, and the effect of BQ\u2010123 (a special ETA receptor antagonist) on the AGML.<\/jats:p><jats:p><jats:bold>METHODS:\u2003<\/jats:bold> A rat model of gastric ulcer induced by cold\u2010restraint\u2010stress (CRS) was used. ET\u20101 concentrations in the plasma and gastric mucosa were determined by radioimmunoassay (RIA), gastric mucosa blood flow (GMBF) was measured with a laser Doppler flow meter, the ulcer index (UI) was used to estimate the degree of gastric mucosa damage and the expression levels of ET\u20101 mRNA in the gastric mucosa were measured using dot blot and reverse transcription polymerase chain reaction (RT\u2010PCR). Different doses of BQ\u2010123 were administered via the left femoral vein prior to the stress in order to observe the effects of BQ\u2010123 on the ET\u20101 concentrations in the plasma and gastric mucosa, the GMBF and the UI.<\/jats:p><jats:p><jats:bold>RESULTS:\u2003<\/jats:bold> Compared with the normal controls, the ET\u20101 concentrations in the plasma and gastric mucosa of the stressed rats were increased significantly (<jats:italic>P<\/jats:italic>\u00a0&lt; 0.05), the GMBF was decreased markedly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and the UI increased dramatically (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). There was a significant positive correlation between the gastric mucosal ET\u20101 concentration and the UI (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.98, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and a significant negative correlation between the gastric mucosal ET\u20101 concentration and GMBF (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.89, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and also between the UI and GMBF (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.98, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). The expression level of ET\u20101 mRNA in the gastric mucosa of the stressed rats increased significantly compared with that of the normal controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and there was a positive correlation between the expression of ET\u20101 mRNA and the ET\u20101 concentration in the gastric mucosa (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.93, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Compared with the untreated animals, the GMBF was increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and the UI decreased significantly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) in the BQ\u2010123\u2010treated rats, and the dose of BQ\u2010123 correlated with the degree of change in the GMBF and UI; however, the ET\u20101 concentrations of either the plasma or the gastric mucosa did not change markedly in the BQ\u2010123\u2010treated animals (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05).<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2003<\/jats:bold> The present study showed that the level of expression of ET\u20101 mRNA and the synthesis of ET\u20101 in the gastric mucosa both increased significantly, which suggests that the increased concentration of endogenous ET\u20101 may be involved in the development and progression of stress ulcer (acute gastric mucosa lesion). The mechanism of action may be associated with a reduction of GMBF induced by ETAR\u2010mediated vasoconstriction. BQ\u2010123 can dose\u2010dependently attenuate significantly the degree of damage to the gastric mucosa induced by stress, and may have therapeutic benefits for stress ulcer.<\/jats:p>","DOI":"10.1111\/j.1443-9573.2004.00149.x","type":"journal-article","created":{"date-parts":[[2004,2,10]],"date-time":"2004-02-10T10:09:05Z","timestamp":1076407745000},"page":"28-34","source":"Crossref","is-referenced-by-count":5,"title":["Changes in endothelin\u20101 gene expression in the gastric mucosa of rats under cold\u2010restraint\u2010stress"],"prefix":"10.1111","volume":"5","author":[{"given":"Yi Min","family":"Duan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao Shen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian Bao","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo Ming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen Xing","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Fang Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,10]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"L192","article-title":"Endothelin\u20101 synthesis, receptors, and signal transduction in alveolar epithelim: evidence for an autocrine role","volume":"268","author":"Markewitz BA","year":"1995","journal-title":"Am J Physiol"},{"key":"e_1_2_5_3_2","first-page":"677","article-title":"Study on immuno\u2010histochemical and radioimmunoassay of ET\u20101 in gastric mucosa","volume":"27","author":"Duan YM","year":"2002","journal-title":"Med J Chin PLA"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.81.3.278"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.3.344"},{"key":"e_1_2_5_6_2","first-page":"325","article-title":"Endothelins: molecular biology, biochemistry, pharmacology, physiology, and pathophysiology","volume":"46","author":"Rubanyi GM","year":"1994","journal-title":"Pharmacol Rev"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200036051-00004"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018829718952"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022864120761"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199007053230106"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(97)90335-4"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365529850171017"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2000.02061.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-4179(97)00007-7"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02936362"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1999.00516.x"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2000.02142.x"}],"container-title":["Chinese Journal of Digestive Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1443-9573.2004.00149.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1443-9573.2004.00149.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T13:52:43Z","timestamp":1697205163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1443-9573.2004.00149.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1111\/j.1443-9573.2004.00149.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1443-9573.2004.00149.x","archive":["Portico"],"relation":{},"ISSN":["1443-9611","1443-9573"],"issn-type":[{"value":"1443-9611","type":"print"},{"value":"1443-9573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2]]}}}