{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T12:55:16Z","timestamp":1783947316690,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,7,9]],"date-time":"2004-07-09T00:00:00Z","timestamp":1089331200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chinese Journal of Digestive Diseases"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p><jats:bold>OBJECTIVE:\u2003<\/jats:bold> Visceral hypersensitivity is highly prevalent in most functional bowel disorders, such as irritable bowel syndrome (IBS), and activation of intestinal mast cells (MC) may play a role because they have been found in close proximity to gastrointestinal mucosal sensory nerve terminals containing neuropeptides and a bi\u2010directional pathway connecting the central nervous system, gut, and MC has been demonstrated. The current study appraised the status of rectal visceral perception, as well as the changes in the MC and substance P (SP) in the intestinal mucosa of patients with IBS.<\/jats:p><jats:p><jats:bold>METHODS:\u2003<\/jats:bold> The study group comprised 42 patients with IBS and 19 healthy subjects who underwent anorectal manometry and rectal perception thresholds to balloon distension. The MC and the SP\u2010ergic terminals in the mucosa were stained for respective histochemical and immunohistochemical investigations. The results were presented both qualitatively and quantitatively by color image analyzer, based on analysis of the intensity and area of stained fibrils. The structural relationship between the MC and nerve terminals was studied by electron microscopy, using an <jats:italic>in situ<\/jats:italic> embedding technique.<\/jats:p><jats:p><jats:bold>RESULTS:\u2003<\/jats:bold> The anorectal resting pressure, squeezing pressure and relaxation pressure were normal in both groups. The sensation threshold, defecation threshold and pain threshold in diarrhea\u2010predominant IBS and the pain thresholds in constipation\u2010predominant IBS were much lower than in the controls. Rectal compliance decreased in IBS. The number of MC in the terminal ileum, the ileocecal junction and the ascending colon was significantly elevated in IBS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and the MC showed great variation. A significantly increased concentration of SP was found in the colon of the IBS patients compared with the controls. There was a positive correlation between the profiles of mucosal MC and the SP\u2010ergic terminals, and MC were closely adjacent to SP\u2010ergic terminals in the lamina propria.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2003<\/jats:bold> As altered rectal perception is present in almost all patients with IBS, it might be a reliable biological characteristic of the disease. Alterations in the MC and SP of the intestinal mucosa may be important factors in visceral hypersensitivity.<\/jats:p>","DOI":"10.1111\/j.1443-9573.2004.00168.x","type":"journal-article","created":{"date-parts":[[2004,7,9]],"date-time":"2004-07-09T12:02:16Z","timestamp":1089374536000},"page":"103-109","source":"Crossref","is-referenced-by-count":29,"title":["Study of visceral hypersensitivity in irritable bowel syndrome"],"prefix":"10.1111","volume":"5","author":[{"given":"Wen Zhu","family":"DONG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duo Wu","family":"ZOU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao Shen","family":"LI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Ping","family":"ZOU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai Yong","family":"ZHU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo Ming","family":"XU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"YIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Fang","family":"GONG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen Xing","family":"SUN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Hua","family":"MAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,9]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1385\/MN:24:1-3:183"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024340"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1998.tb00558.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000050681"},{"key":"e_1_2_5_6_2","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.4049\/jimmunol.163.5.2410","article-title":"Direct neurite\u2010mast cell communication in vitro occurs via the neuropeptide substance P","volume":"163","author":"Suzuki R","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_5_7_2","first-page":";","volume-title":"Rome II: the Functional Gastrointestinal Disorders","author":"Drossman DA","year":"2000"},{"key":"e_1_2_5_8_2","first-page":";","volume-title":"The Section's Fabrication of Pathological Constitution","author":"Zhijin G","year":"1994"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.7.506"},{"key":"e_1_2_5_10_2","first-page":"79","article-title":"Light and electron microscopic study of the relationship of the substance P and vasoactive intestinal polypeptide immunoreactive nerve terminals with mast cells on meridian line in human skin","volume":"2","author":"Wang JM","year":"1986","journal-title":"J Neuroanat"},{"key":"e_1_2_5_11_2","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1016\/S0002-9270(01)02515-1","article-title":"Visceral afferent hypersensitivity in irritable bowel syndrome: evaluation by cerebral evoked potential after rectal stimulation","volume":"96","author":"Sinhamahapatra P","year":"2001","journal-title":"AmJ Gastroenterol"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(01)00285-8"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.6.814"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.33601"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2001.03804.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01303164"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.2000.00221.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2002.01185.x"},{"key":"e_1_2_5_19_2","first-page":"231","article-title":"Mast cells of ileocecal junction in irritable bowel syndrome","volume":"36","author":"Yang Y","year":"1997","journal-title":"Chung Hua Nei Ko Tsa China"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1995.tb07303.x"},{"key":"e_1_2_5_21_2","first-page":"91","article-title":"Innervation of mucosal immune cells in the gastrointestinal tract","volume":"4","author":"Stead RH","year":"1992","journal-title":"Reg Immunol"},{"key":"e_1_2_5_22_2","doi-asserted-by":"crossref","first-page":"G315","DOI":"10.1152\/ajpgi.2001.280.3.G315","article-title":"Stress and the gastrointestinal tract. 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