{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:16:09Z","timestamp":1783462569431,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,10,17]],"date-time":"2006-10-17T00:00:00Z","timestamp":1161043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chinese Journal of Digestive Diseases"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p><jats:bold>OBJECTIVE:\u2003<\/jats:bold> Irritable bowel syndrome (IBS) is a common disorder in clinical practice, but the pathophysiology of IBS has not been completely elucidated yet. Experiments have revealed that the concentrations of some kinds of brain\u2013gut peptides, such as substance P, were abnormal in the plasma and\/or the intestinal mucosa. In order to explore further the possible role of substance P containing nerve fibers in the enteric nervous system and central nervous system, the expression of c\u2010fos, a well\u2010established marker of activated neural pathway, was induced to show substance P containing a neural pathway in the rat model of constipation\u2010predominant IBS by rectal distention.<\/jats:p><jats:p><jats:bold>METHODS:\u2003<\/jats:bold> The rat model was set up by intragastric instillation of 2.0\u00a0mL water at 0\u20134\u00b0C in 20 male Wistar rats for two weeks. Both the model group and the controls underwent rectal distention under deep anesthesia. Sections containing the anatomical areas of interest were obtained and processed for c\u2010fos protein and substance P immunohistochemistry using the strept avidin\u2010biotin complex (SABC) method. The staining results were analyzed semi\u2010quantitatively, using a computerized color image analyzer with two parameters: opacity density and immunoreactive areas. The statistical difference of the opacity density and immunoreactive areas between the two groups was analyzed by a <jats:italic>t<\/jats:italic>\u2010test. Correlation analysis was used to investigate the relationship between the expression of substance P and c\u2010fos protein of the same region in the model group.<\/jats:p><jats:p><jats:bold>RESULTS:\u2003<\/jats:bold> The opacity density of substance P immunoreactive tissues in the ileocecal junction, colon, the posterior horn of the spinal cord and the hypothalamus of the model group were all significantly higher compared with those in the control group (176.6 vs 155.5, 172.3 vs 152.0, 182.1 vs 160.2, 128.3 vs 117.9; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05, respectively). Meanwhile in the ileocecal junction, colon, the posterior horn of the spinal cord and the hypothalamus of the model group, the opacity density of c\u2010fos protein\u2010positive tissue were all significantly higher than those of the same region in the controls (120.9 vs 109.0, 101.3 vs 92.2, 125.4 vs 88.7, 115.5 vs 88.6; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05, respectively). The distribution of c\u2010fos protein\u2010positive tissue is similar to that of the substance P and the analysis shows that there is close correlation between the expression of substance P and c\u2010fos protein of the same region in the model group (<jats:italic>r\u00a0=<\/jats:italic>\u00a00.594\u20130.721, <jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00.05).<\/jats:p><jats:p><jats:bold>CONCLUSIONS:\u2003<\/jats:bold> The expression of substance P and c\u2010fos protein in both the enteric nervous system and the central nervous system of the constipation\u2010predominant IBS rat model is abnormal, which suggests that an abnormal change in substance P may be involved in the pathogenesis of IBS and the substance P\u2010containing neural pathway may be one of the neural pathways that play important role in the regulation of the gastrointestinal function.<\/jats:p>","DOI":"10.1111\/j.1443-9573.2006.00273.x","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T09:28:02Z","timestamp":1161163682000},"page":"211-218","source":"Crossref","is-referenced-by-count":16,"title":["Alternation of substance P\u2010containing neural pathways in a rat model of irritable bowel syndrome with rectal distension"],"prefix":"10.1111","volume":"7","author":[{"given":"Wei Feng","family":"WANG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Sheng","family":"YANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Hua","family":"PENG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"SUN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,17]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"320","article-title":"The regulating mechanism of nerve\u2010immunity\u2010 endocrine net in irritable bowel syndrome","volume":"11","author":"Yang YS","year":"2002","journal-title":"Chinese J Gastroenterology and Hepatology"},{"key":"e_1_2_5_3_2","first-page":"112","article-title":"A new rat model of constipation\u2010predominant irritable bowel syndrome","volume":"12","author":"Peng LH","year":"2004","journal-title":"World J Gastroenterology"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)04197-5"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00465-8"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.2741\/1203"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/102538902900012369"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0954-6820.2000.00773.x"},{"key":"e_1_2_5_9_2","first-page":"437","article-title":"Cholecystokinin and substance P of the plasma and sigmoid mucosa in irritable bowel syndrome","volume":"5","author":"Yang YS","year":"1997","journal-title":"World J Gastroenterol"},{"issue":"116","key":"e_1_2_5_10_2","first-page":"121","article-title":"The changes of gut hormones in irritable bowel syndrome","volume":"6","author":"Wang YK","year":"2001","journal-title":"Chinese J Gastroenterology"},{"key":"e_1_2_5_11_2","first-page":"147","article-title":"Changes of colonic substance P, vasoactive intestinal polypeptide and calcitonin gene\u2010related peptide in irritable bowel syndrome","volume":"24","author":"Li ZS","year":"2003","journal-title":"Academic J Second Military Medical University"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199612000-00014"},{"key":"e_1_2_5_13_2","first-page":"35","article-title":"Immunohistochemical study of VIP and SP on the colonic wall in patients with slow transit constipation","volume":"18","author":"Gao F","year":"1998","journal-title":"Chinese J Digestion"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.6.804"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)82639-6"},{"key":"e_1_2_5_16_2","first-page":"231","article-title":"Mast cells of ileocecal junction in irritable bowel syndrome","volume":"36","author":"Yang YS","year":"1997","journal-title":"Chinese J Internal Medicine"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.031310"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.279.1.G139"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.51.suppl_1.i41"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000508)420:3<349::AID-CNE6>3.0.CO;2-K"},{"key":"e_1_2_5_21_2","first-page":"195","article-title":"Substance P: transmitter of nociception (Mini review)","volume":"34","author":"Zubrzycka M","year":"2000","journal-title":"Endocr Regul"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025455330704"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70170-3"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.6.G1512"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70220-8"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.05545.x"},{"key":"e_1_2_5_27_2","first-page":"161","article-title":"Role of substance P in central control of ovulation in female rats","volume":"33","author":"Potargowicz E","year":"1999","journal-title":"Endocr Regul"},{"key":"e_1_2_5_28_2","first-page":"1437","article-title":"TAK\u2010637. Takeda","volume":"2","author":"Furness JB","year":"2001","journal-title":"Curr Opin Investig Drugs"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(99)00006-4"},{"key":"e_1_2_5_30_2","first-page":"87","article-title":"Theoretical aspects of neuroplasticity","volume":"48","author":"Trojan S","year":"1999","journal-title":"Physiol Res"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004691-200003000-00004"}],"container-title":["Chinese Journal of Digestive Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1443-9573.2006.00273.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1443-9573.2006.00273.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T03:28:38Z","timestamp":1696908518000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1443-9573.2006.00273.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,17]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1111\/j.1443-9573.2006.00273.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1443-9573.2006.00273.x","archive":["Portico"],"relation":{},"ISSN":["1443-9611","1443-9573"],"issn-type":[{"value":"1443-9611","type":"print"},{"value":"1443-9573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,17]]}}}