{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:32:53Z","timestamp":1787941973345,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,5,19]],"date-time":"2004-05-19T00:00:00Z","timestamp":1084924800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Internal Medicine Journal"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p> <jats:bold>Abstract<\/jats:bold> <\/jats:p><jats:p><jats:bold>Background<\/jats:bold> :\u2002The measurement of serum antibodies to <jats:italic>Campylobacter<\/jats:italic> spp. has been used to investigate links between prior <jats:italic>Campylobacter<\/jats:italic> infections and the development of Guillain\u2212Barre syndrome and its variants. Little is known of the serum antibody response to acute infections in the short\u2010 or long\u2010term.<\/jats:p><jats:p><jats:bold>Aims<\/jats:bold> : The aims of the present study were to investigate the normal serum response to an acute <jats:italic>Campylobacter<\/jats:italic> infection and the sensitivity and specificity of anti\u2010<jats:italic>Campylobacter<\/jats:italic> antibodies in determining recent <jats:italic>Campylo\u00adbacter<\/jats:italic> infection.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> :\u2002An enzyme\u2010linked immunosorbent assay (ELISA) method was used to measure serum anti\u2010<jats:italic>Campylobacter<\/jats:italic> immunoglobulin\u2003G (IgG), IgA and IgM antibodies. Controls consisted of 420 blood donors without recent gastroenteritis, 25 patients with other gastrointestinal infections, 24 patients with neurological conditions not affecting the peripheral nerves and 19 patients with autoimmune disorders. Three patient groups were assessed: 99 patients with acute <jats:italic>Campy\u00adlo\u00adbacter<\/jats:italic> infections, all of whom were tested 3\u2003weeks post\u2010infection; 69 of these patients tested 3\u22126\u2003months later; and 74 additional patients tested&gt;20\u2003months post\u2010\u00adinfection. Western blot analysis was performed on controls and patients with high titre anti\u2010<jats:italic>Campylobacter<\/jats:italic> anti\u00adbodies to assess for cross\u2010reactivity and specificity.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> :\u2002Following acute infections, all antibody classes rose in the majority of but not in all patients, followed by decreasing titres that did not return to baseline levels. Sixteen per cent of enteritis cases did not demonstrate a rise in titres and 9% of cases had significant levels of antibodies&gt;20\u2003months post\u2010infection. The ELISA used was shown to be highly specific for the detection of <jats:italic>Campylobacter<\/jats:italic> antibodies.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> :\u2002The use of <jats:italic>Campylobacter<\/jats:italic>\u2010specific antibody levels as the sole marker of prior infection is an unreli\u00adable method of determining the association between <jats:italic>Campylobacter<\/jats:italic> infection and neurological disease. (Intern Med J 2004; 34: 250\u2212258)<\/jats:p>","DOI":"10.1111\/j.1444-0903.2004.00517.x","type":"journal-article","created":{"date-parts":[[2004,5,19]],"date-time":"2004-05-19T10:00:11Z","timestamp":1084960811000},"page":"250-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Sensitivity and specificity of serology in determining recent acute <i>Campylobacter<\/i> infection"],"prefix":"10.1111","volume":"34","author":[{"given":"B. 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