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Clinical trials suggest that cyclooxygenase (COX) inhibitors might prevent oesophageal cancer, although PPIs could offset this by increasing COX\u20102 expression in Barrett's oesophagus. To investigate this, we evaluated the impact of a PPI on COX expression in oesophageal mucosal cells.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>The effect of the PPI esomeprazole on COX\u20101 and COX\u20102 mRNA levels in oesophageal cells was determined. Oesophageal cell lines OE33 (adenocarcinoma\u2010derived) and HET\u20101A (immortalized squamous cells) and a control intestinal cell line HT29 (colon carcinoma) were treated for 24\u2003h, with increasing concentrations of the esomeprazole.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>COX\u20102, but not COX\u20101, mRNA levels dose\u2010dependently increased in OE33 and HET\u20101A cells versus esomeprazole concentration. COX\u20102 mRNA levels did not increase in HT29 cells.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>Exposure to esomeprazole increases COX\u20102 mRNA in oesophageal cells. This might contribute to the lack of benefit for COX inhibitors for oesophageal cancer prevention in recent clinical studies.<\/jats:p>","DOI":"10.1111\/j.1445-2197.2012.06124.x","type":"journal-article","created":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T14:20:15Z","timestamp":1341325215000},"page":"691-696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["COX\u20102 mRNA is increased in oesophageal mucosal cells by a proton pump inhibitor"],"prefix":"10.1111","volume":"82","author":[{"given":"George C.","family":"Mayne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David I.","family":"Watson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian 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