{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T20:30:39Z","timestamp":1774470639459,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> Rapid detection of fetal aneuploidy helps inform a mother\u2019s choice about the course of her pregnancy. Obtaining results by fluorescent <jats:italic>in situ<\/jats:italic> hybridization (FISH) requires more than 24\u2003h, and thus a more rapid method is needed.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Conventional G\u2010banding and FISH for chromosome 21 were performed for cultured amniocytes. Genomic DNA was extracted from uncultured amniocytes obtained from 23 patients. TaqMan polymerase chain reaction (PCR) primers were designed to amplify the potassium voltage gated channel gene on chromosome 21q22.12 and the ribosomal phosphoprotein gene on 18q21.1. Quantitative real\u2010time PCR was performed for these two gene fragments and the differences of the threshold cycle (Ct) of the two genes (Ct 18\u2013Ct 21) were calculated for each sample.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> G\u2010banding revealed that 19 patients had a normal karyotype and four had trisomy 21. FISH resulted in one case of a false positive. The \u0394Ct values (Ct 18\u2013Ct 21) of trisomy 21 patients were significantly higher than the values of individuals with normal karyotypes (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) and there was no overlapping.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Fetal trisomy 21 is rapidly detectable by gene dosage analysis from amniocytes using quantitative real\u2010time PCR.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2006.00428.x","type":"journal-article","created":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T10:32:35Z","timestamp":1153132355000},"page":"368-372","source":"Crossref","is-referenced-by-count":8,"title":["Rapid detection of trisomy 21 by gene dosage analysis using quantitative real\u2010time polymerase chain 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