{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T04:12:35Z","timestamp":1742703155857,"version":"3.40.2"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,2]],"date-time":"2012-03-02T00:00:00Z","timestamp":1330646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold>The aim of the present study was to evaluate the effect of lipopolysaccharide (LPS) on the production of prostaglandin E2 (PGE2) and inhibition by nitric oxide (NO) of spontaneous contractions of uterine rings from pregnant inducible isoform of nitric oxide synthase knockout (iNOS KO) mice.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold>iNOS KO and wild\u2010type mice were sacrificed 6\u2003h after intraperitoneal (i.p.) administration of LPS on day 14 of gestation. Uterine rings were equilibrated in Krebs\u2010Henseleit solution for isometric tension recording. In part of the uterine rings, placental tissues were left attached. The bathing solution was analyzed for PGE2 by radioimmunoassay. Changes in spontaneous contractions in response to cumulative concentrations of L\u2010arginine, diethylamine\/nitric oxide (DEA\/NO), and 8\u2010bromo\u2010cyclic guanosine monophosphate (8\u2010br\u2010cGMP) were determined.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>Treatment with LPS increased PGE2 production by uterine rings from wild\u2010type and iNOS\u2010KO mice. DEA\/NO and 8\u2010br\u2010cGMP inhibited spontaneous contractions in uterine rings in the absence or presence of placenta, in both LPS\u2010treated and LPS\u2010untreated animals. LPS treatment attenuated maximal inhibition induced by the agents, both in the absence and presence of placental tissues in iNOS KO and wild\u2010type mice.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold>LPS induces PG production in mice myometrium that is not dependent on the integrity of iNOS, while LPS could induce pathophysiological iNOS obstruct uterine quiescence by physiological iNOS. Infection affects uterine contractile activity through PG production, as well as through placental and genetic factors. NO may be a double\u2010edged sword in pregnant mice myometrium.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2011.01772.x","type":"journal-article","created":{"date-parts":[[2012,3,4]],"date-time":"2012-03-04T08:27:28Z","timestamp":1330849648000},"page":"714-720","source":"Crossref","is-referenced-by-count":1,"title":["Effect of lipopolysaccharide on the production of prostaglandin E2 and inhibition of uterine contractions by nitric oxide in pregnant inducible nitric oxide synthase knockout 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