{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:53:00Z","timestamp":1784123580803,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,8]],"date-time":"2012-05-08T00:00:00Z","timestamp":1336435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold>Shakuyaku\u2010kanzo\u2010to (SK) is a herbal medicine and is known to possess an antispasmodic effect on skeletal muscle and intestinal smooth muscle. However, it is unclear whether SK is effective in antagonizing uterine smooth muscle contractions. Herein, we investigated the effects of SK on smooth muscle contractions of human pregnant uterine samples.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold>We prepared myometrial strips from uterine tissues of pregnant women who underwent cesarean section for obstetrical indications, and examined the inhibitory effects of SK and its components, shakuyaku (S) and kanzo (K), on agonist\u2010induced and spontaneous contractions<jats:italic>in vitro<\/jats:italic>. Oxytocin, prostaglandinF<jats:sub>2\u03b1<\/jats:sub>, and high KCl were utilized as agonists in this study.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>SK inhibited agonist\u2010induced and spontaneous contractions in a dose\u2010dependent manner. Inhibition of SK on oxytocin\u2010induced contractions occurred at a concentration of 100\u2003\u00b5g\/mL and reached maximum effect at a concentration of more than 1000\u2003\u00b5g\/mL. The half max inhibitory concentration of SK was approximately 440\u2003\u00b5g\/mL in oxytocin\u2010induced contractions. SK at 1000\u2003\u00b5g\/mL completely inhibited the oxytocin\u2010 and prostaglandinF<jats:sub>2\u03b1<\/jats:sub>\u2010induced contractions but not the high KCl\u2010induced contractions. The inhibitory effects on agonist\u2010induced contractions of K, but not S, matched those of SK.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold>These results suggest that the inhibitory effect of SK on smooth muscle contractions is due to K. The mechanism of the inhibitory effects of SK on oxytocin\u2010 and prostaglandinF<jats:sub>2\u03b1<\/jats:sub>\u2010induced contractions may differ from that on KCl\u2010induced contractions.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2011.01827.x","type":"journal-article","created":{"date-parts":[[2012,5,9]],"date-time":"2012-05-09T03:41:56Z","timestamp":1336534916000},"page":"1004-1010","source":"Crossref","is-referenced-by-count":17,"title":["Shakuyaku\u2010kanzo\u2010to inhibits smooth muscle contractions of human pregnant uterine tissue<i>in vitro<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"Shoko","family":"Tsuji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genichiro","family":"Sumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisayuu","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Tsuzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideharu","family":"Kanzaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X03001144"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000049057"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000093256"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i5.760"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-008-0304-6"},{"key":"e_1_2_7_7_2","first-page":"163","article-title":"Possible 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