{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:30:37Z","timestamp":1767864637574,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,6,4]],"date-time":"2012-06-04T00:00:00Z","timestamp":1338768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> MicroRNA\u201034a (miR\u201034a) is associated with invasion and metastasis of various cancers. The trophoblastic cells of placenta accreta invade into the myometrium in a similar way to the invasion of cancers. We studied the roles of miR\u201034a in the pathogenesis of placenta accreta.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The human choriocarcinoma cell line JAR was used for in vitro experiments as a model of trophoblasts, and placental tissues from the operative specimen of patients with or without placenta accreta were used for experiments in vivo. Morpholino antisense oligomer against miR\u201034a (miR\u201034a Morpho\/AS) was added to JAR, and the expression of miR\u201034a and plasminogen activator inhibitor\u20101 (<jats:italic>PAI\u20101<\/jats:italic>) was determined by real time PCR. The effects of antisense, interleukin (IL)\u20106 and IL\u20108 in the process of invasion were studied with an invasion assay. Expression of miR\u201034a in vivo was studied with the use of fluorescent in situ hybridization (FISH).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Expression of miR\u201034a was inhibited by 65% with the administration of antisense, and a slight increase in miR\u201034a expression was observed with the addition of IL\u20106 and IL\u20108. <jats:italic>PAI\u20101<\/jats:italic> expression decreased with the addition of IL\u20106 and IL\u20108, and increased with the administration of antisense. There was an increase in invasive capacity through the inhibition of miR\u201034a expression. Strong FISH expression of miR\u201034a was observed in trophoblast cells of non\u2010placenta accreta, and a clear decrease in miR\u201034a expression was observed in those of placenta accreta.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Expression of miR\u201034a was downregulated in placenta accreta. In vitro experiments also showed that the invasive potential of JAR increased by suppressing miR\u201034a, probably through the expression of <jats:italic>PAI\u20101<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2012.01898.x","type":"journal-article","created":{"date-parts":[[2012,6,4]],"date-time":"2012-06-04T18:11:19Z","timestamp":1338833479000},"page":"67-74","source":"Crossref","is-referenced-by-count":38,"title":["Roles of microRNA\u201034a in the pathogenesis of placenta 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