{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T19:27:43Z","timestamp":1750188463026},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,8,13]],"date-time":"2012-08-13T00:00:00Z","timestamp":1344816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> The aim of this study was to explore the effect of Src\u2010homology\u20102\u2010domain\u2010containing protein, tyrosine phosphatase 2 (SHP\u20102) on the proliferation of cervical cancer cells.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> A total of 45 patients with cervical cancer (stage I\u2013III), 32 with cervical intraepithelial neoplasia and 20 healthy subjects were consecutively recruited. The levels of SHP\u20102 and interferon (IFN)\u2010\u03b2 expression in cervical tissues were characterized by immunohistochemistry and statistically analyzed by logistic regression. Following knockdown of SHP\u20102 expression by a siRNA or pre\u2010treatment with a specific peptide, the effect of SHP\u20102 expression in THP\u20101 cells on the growth and survival of SiHa cells and on IFN\u2010\u03b2 production was determined by co\u2010culture assays, 3\u2010(4,5)\u2010dimethylthiazol (\u2010z\u2010y 1)\u20103,5\u2010diphenyltetrazolium bromide, and enzyme immunosorbent assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The levels of SHP\u20102 expression in cervical cancer tissues were significantly higher than that in cervical intraepithelial neoplasia and uterine myoma tissues (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05, respectively), and negatively correlated with the levels of IFN\u2010\u03b2 expression in these tissues (R\u2003=\u2003\u22120.582, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Knockdown of SHP\u20102 expression with SHP\u20102 siRNA or treatment with the SHP\u20102\u2010specific blocking peptide in THP\u20101 cells significantly increased the production of IFN\u2010\u03b2 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05, respectively) and inhibited the proliferation of SiHa cells in a co\u2010culture system of THP\u20101 and SiHa cells (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05, respectively).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> SHP\u20102 phosphatase promotes cervical cancer cell proliferation through inhibiting IFN\u2010\u03b2 production.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2012.01952.x","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T01:42:23Z","timestamp":1344908543000},"page":"272-279","source":"Crossref","is-referenced-by-count":9,"title":["SHP\u20102 phosphatase promotes cervical cancer cell proliferation through inhibiting interferon\u2010\u03b2 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