{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:48:49Z","timestamp":1787669329322,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,26]],"date-time":"2012-08-26T00:00:00Z","timestamp":1345939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> Hypoxia, a characteristic of almost all types of solid tumors, has been associated with poor outcome in a number of human malignancies. The aim of this study was to investigate the molecular mechanisms involved in hypoxia\u2010induced activation of the human survivin gene promoter in cervical HeLa cells.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Immunohistochemical staining was used to detect the expression of HIF\u20101\u03b1 and survivin in cervical cancer samples and normal cervical samples. Under normoxic and hypoxic conditions, the expression of hypoxia inducible factor (HIF)\u20101\u03b1 and survivin in cervical cancer HeLa cells was detected by quantitative reverse transcription polymerase chain reaction and Western blotting. Luciferase reporter assays was used to investigate the molecular mechanisms in hypoxia\u2010induced survivin activation. We also studied the effect of HIF\u20101\u03b1 overexpression on the expression of survivin in cervical cancer HeLa cells.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Significant HIF\u20101\u03b1 and survivin overexpression is associated with cervical cancer, and HIF\u20101\u03b1 protein expression is strongly correlated with survivin protein expression. In cervical cancer cell line (HeLa), hypoxia upregulated both HIF\u20101\u03b1 and survivin expression. Moreover, luciferase reporter assays using survivin core promoter demonstrated that survivin transcription was activated under hypoxia conditions and was associated with HIF\u20101\u03b1 overexpression. The transcriptional activation of reporter genes in response to hypoxia is independent of potential HIF\u20101\u03b1\u2010responsive element, located between \u221286 and \u221282 regions. HIF\u20101\u03b1 overexpression significantly activated survivin expression.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our results demonstrate that survivin expression is upregulated following the induction of HIF\u20101\u03b1 by hypoxia resulting from tumor formation, possibly leading to tumor progression. These findings have potential implication in developing novel cancer therapy targeting HIF\u20101.<\/jats:p>","DOI":"10.1111\/j.1447-0756.2012.01995.x","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T19:33:50Z","timestamp":1346096030000},"page":"555-563","source":"Crossref","is-referenced-by-count":24,"title":["Hypoxia inducible factor\u20101\u03b1\u2010mediated activation of survivin in cervical cancer cells"],"prefix":"10.1111","volume":"39","author":[{"given":"Haitao","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengfang","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanxiang","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-007-0281-3"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00538.2003"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7082-2"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1187"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/25141"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10327"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.1228"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1048-891X.2005.15011.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409350200"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)03084-8"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302280"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03343.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64554-3"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.02.140"},{"key":"e_1_2_7_16_2","first-page":"5830","article-title":"Overexpression of hypoxia\u2010inducible factor 1 in common human cancers and their metastases","volume":"59","author":"Zhong H","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/82146"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/28867"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.9.4604"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11373-007-9173-3"},{"key":"e_1_2_7_21_2","first-page":"409","article-title":"Effect of hypoxia\u2010inducible factor 1\u2010\u03b1 on survivin in colorectal cancer","volume":"3","author":"Wu XY","year":"2010","journal-title":"Mol Med Rep"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1413-7"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.25.14517"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207460"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.10.005"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600196"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(00)00142-1"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1447-0756.2012.01995.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1447-0756.2012.01995.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T23:03:04Z","timestamp":1697324584000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/j.1447-0756.2012.01995.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,26]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/j.1447-0756.2012.01995.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1447-0756.2012.01995.x","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,26]]}}}