{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T18:28:23Z","timestamp":1719772103345},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,4,5]],"date-time":"2004-04-05T00:00:00Z","timestamp":1081123200000},"content-version":"vor","delay-in-days":35,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute cocaine administration increases phosphorylation of dopamine and cAMP\u2010regulated phosphoprotein (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>\u200332\u2003kDa) (DARPP\u201032) at threonine (Thr)\u201034, whereas repeated cocaine administration increases DARPP\u201032 phosphorylation at Thr\u201075 in Sprague\u2013Dawley rat striatum. Repeated acetyl\u2010<jats:sc>l<\/jats:sc>\u2010carnitine (ALCAR) administration persistently increases dopamine outflow in the nucleus accumbens. The present study examined the effect of repeated ALCAR administration on the DARPP\u201032 phosphorylation pattern in the nucleus accumbens and caudate\u2010putamen. ALCAR increased phosphoThr\u201034 DARPP\u201032 levels and decreased phosphoThr\u201075 DARPP\u201032 levels, after 1 and 10\u2003days of washout. We compared the effects of repeated cocaine and repeated ALCAR administrations on the behavioural response to cocaine challenge and on the DARPP\u201032 phosphorylation pattern and cyclin\u2010dependent kinase\u20035 (Cdk5) levels in the striatum. We also studied whether ALCAR administered daily during or after cocaine sensitization procedure would interfere with the effects of cocaine. When the response to the cocaine challenge was assessed, cocaine\u2010 and ALCAR\u2010treated rats showed a similar sensitized behavioural response, and rats receiving combined cocaine and ALCAR treatments, irrespective of treatment order, also showed a sensitized response. A week after the cocaine challenge, the two drugs had induced opposite modifications in DARPP\u201032 phosphorylation, as cocaine increased phosphorylation at Thr\u201075, while ALCAR increased phosphorylation at Thr\u201034. In cocaine plus ALCAR treated rats, irrespective of treatment order, ALCAR administration antagonized cocaine effects on DARPP\u201032 phosphorylation. Moreover, cocaine, but not ALCAR, increased \u0394FosB and Cdk5 expression, and the increase in Cdk5 was antagonized by ALCAR administration in rats receiving combined treatments. These effects were relatively persistent, as they were still present 7\u2003days after the last treatment.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2004.03230.x","type":"journal-article","created":{"date-parts":[[2004,4,5]],"date-time":"2004-04-05T07:25:24Z","timestamp":1081149924000},"page":"1609-1620","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Repeated acetyl\u2010<scp>l<\/scp>\u2010carnitine administration increases phospho\u2010Thr34 DARPP\u201032 levels and antagonizes cocaine\u2010induced increase in Cdk5 and phospho\u2010Thr75 DARPP\u201032 levels in rat striatum"],"prefix":"10.1111","volume":"19","author":[{"given":"Simona","family":"Scheggi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Rauggi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulio","family":"Nanni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Tagliamonte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Gambarana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.10232"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/45251"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.001401"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964865"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000107167"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(75)90834-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(88)90319-0"},{"key":"e_1_2_6_9_1","first-page":"61","article-title":"Under continuous dizocilpine infusion an N\u2010methyl\u2010d\u2010aspartate receptor independent form of cocaine sensitization develops in rats","volume":"9","author":"Gambarana C.","year":"1998","journal-title":"Behav. 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