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For this investigation a seizure\u2010specific mouse brain cDNA library was generated and spotted onto microarray slides with the aim of increasing the likelihood of identifying novel genes responsive to seizure. Microarray analysis was performed on mouse hippocampus 1\u2003h after generalized seizure pharmacologically induced by pentylenetetrazol (PTZ). Using the custom microarray slides, six genes were identified as being up\u2010regulated in this seizure model and results were validated by real\u2010time PCR. Four of the seizure\u2010responsive genes had previously\u2010reported roles in apoptosis, proliferation or differentiation of neural cells. Two of the genes were novel and<jats:italic>in situ<\/jats:italic>hybridization analysis demonstrated heightened mRNA expression in the hippocampus 1\u2003h following generalized convulsive seizure, in a pattern which is typical for other activity\u2010dependant genes expressed in this structure. In addition to being up\u2010regulated postseizure, the genes described in this paper appear to be expressed normally in the adult hippocampus and during development.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2004.03646.x","type":"journal-article","created":{"date-parts":[[2004,9,20]],"date-time":"2004-09-20T15:23:01Z","timestamp":1095693781000},"page":"1819-1826","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["<i>Nxf<\/i>and<i>Fbxo33<\/i>: novel seizure\u2010responsive genes in mice"],"prefix":"10.1111","volume":"20","author":[{"given":"Warren D.","family":"Flood","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Moyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Tsykin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant R.","family":"Sutherland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon A.","family":"Koblar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,14]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.1013"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7.6.615"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.12.1853"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6531"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200203250-00011"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9191"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.6025"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-06-02218.2003"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199407000-00032"},{"key":"e_1_2_14_11_1","doi-asserted-by":"crossref","first-page":"14511","DOI":"10.1016\/S0021-9258(18)98716-8","article-title":"Structure and expression of TIS21, a primary response gene induced by growth factors and tumor promoters","volume":"266","author":"Fletcher B.S.","year":"1991","journal-title":"J. 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