{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:06:30Z","timestamp":1765231590335},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,7,15]],"date-time":"2005-07-15T00:00:00Z","timestamp":1121385600000},"content-version":"vor","delay-in-days":44,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monitoring multiple neurons is essential for understanding neuronal network activities. While calcium imaging from a population of cells is an effective method to study the network dynamics of a neural structure, it has been difficult to image from densely packed structures, such as the granule cell layer of the dentate gyrus, due to overlap of the cells. We have developed a novel method to label multiple granule cells with a Ca<jats:sup>2+<\/jats:sup> indicator in rat hippocampal slices using Oregon Green 488\u2003BAPTA\u20101\u2003(OGB\u20101)\u2003AM. Synchronized burst activities (0.3\u20131.4\u2003Hz), which were induced by applying 50\u2003\u00b5<jats:sc>m<\/jats:sc> 4\u2010aminopyridine, were monitored extracellularly with a glass electrode placed at the granule cell layer in the dentate gyrus. During the burst activities, spontaneously occurring action potential\u2010induced Ca<jats:sup>2+<\/jats:sup> transients from multiple (4\u201312) granule cells were monitored with a cooled CCD camera with single\u2010cell resolution. Temporal structures of firing patterns from the multiple neurons were determined from Ca<jats:sup>2+<\/jats:sup> transients. In each single\u2010burst\u2010event recorded from the extracellular electrode, each neuron fired synchronously within a 200\u2003ms time window. The latency and its variance from the onset time of the single\u2010burst\u2010events to one of the Ca<jats:sup>2+<\/jats:sup> transients decreased over time (&lt;\u20037.5\u2003min). These results indicate that the synchrony of the action potentials within a single\u2010burst\u2010event was enhanced as the burst activities proceeded. This progressive synchronization may be a key feature in making self\u2010organizing neuronal networks.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2005.04167.x","type":"journal-article","created":{"date-parts":[[2005,7,15]],"date-time":"2005-07-15T17:35:30Z","timestamp":1121448930000},"page":"3349-3360","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Optical monitoring of progressive synchronization in dentate granule cells during population burst activities"],"prefix":"10.1111","volume":"21","author":[{"given":"Masanori","family":"Murayama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Kudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Miyakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6328653"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-12-03912.1996"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-23-09308.1997"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(80)90049-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)91483-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.6.2553"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902460403"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903020203"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(68)90234-5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1063(1999)9:2<137::AID-HIPO5>3.0.CO;2-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00190-8"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-09-04072.1993"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-12-05381.2003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80314-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.191bz.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/74823"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.013bl.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/47035"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79653-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.450040405"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/43119"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-05-01612.2003"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/357244a0"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030110210"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1995.75.4.689"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019965"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0497p.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-7952(06)80019-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2003.09.007"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81085-5"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.79.3.1518"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/7.6.523"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5207"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00237147"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(84)90012-5"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90074-N"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1991.65.1.141"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81125-3"},{"key":"e_1_2_7_40_1","first-page":"275","article-title":"The morphology of synapses in the central nervous system","volume":"14","author":"Palay S.L.","year":"1958","journal-title":"Exp. Cell Res."},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(91)90121-F"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00391.x"},{"key":"e_1_2_7_43_1","first-page":"43.41","volume-title":"Imaging Neurons: a Laboratory Manual","author":"Ross W.N.","year":"1999"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-10-06041.1994"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90100-I"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80993-9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.84.2.927"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1992.67.3.715"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1974.37.3.541"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970901)385:3<427::AID-CNE7>3.0.CO;2-5"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00506-2"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-14-04438.1996"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-12-04860.2002"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1460-9568.2005.04167.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1460-9568.2005.04167.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T02:36:39Z","timestamp":1692239799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1460-9568.2005.04167.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6]]},"references-count":52,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["10.1111\/j.1460-9568.2005.04167.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1460-9568.2005.04167.x","archive":["Portico"],"relation":{},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6]]},"assertion":[{"value":"2005-07-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}