{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:27:49Z","timestamp":1791044869081,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,5,16]],"date-time":"2006-05-16T00:00:00Z","timestamp":1147737600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In mammals, the master circadian clock resides in the suprachiasmatic nuclei (SCN) of the hypothalamus. The period and phase of the circadian pacemaker are calibrated by direct photic input from retinal ganglion cells (RGCs). SCN\u2010projecting RGCs respond to light in the absence of rod\u2010 and cone\u2010driven synaptic input, a property for which they are termed intrinsically photosensitive. In SCN\u2010projecting RGCs, light activates a nonselective cationic current that displays inward and outward rectification. The goal of the present study was to investigate the identity of the light\u2010activated ion channel and the intracellular signaling pathway leading to its activation. We considered two candidate channels, cyclic nucleotide\u2010gated (CNG) channels and transient receptor potential (TRP) channels, which mediate vertebrate and invertebrate phototransduction, respectively. We report that the intrinsic light response relies upon a G\u2010protein\u2010dependent process. Although our data indicate that cyclic nucleotides modulate the signaling pathway, CNG channels do not appear to conduct the light\u2010activated current because (i) cyclic nucleotides in the pipette solution do not activate a conductance or completely block the light response, (ii) CNG channel blockers fail to inhibit the light response, (iii) the effects of internal and external divalent cations are inconsistent with their effects on CNG channels, and (iv) immunohistochemistry reveals no CNG channels in SCN\u2010projecting RGCs. Finally, we show that the pharmacology of the light\u2010activated channel resembles that of some TRPC channel family members; the response is blocked by lanthanides and ruthenium red and SK&amp;F 96365, and is enhanced by flufenamic acid and 1\u2010oleoyl\u20102\u2010acetyl\u2010<jats:italic>sn<\/jats:italic>\u2010glycerol. Furthermore, immunohistochemical experiments reveal that TRPC6 is expressed in many RGCs, including those that express melanopsin.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.04777.x","type":"journal-article","created":{"date-parts":[[2006,5,16]],"date-time":"2006-05-16T13:31:44Z","timestamp":1147786304000},"page":"2477-2487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["The light\u2010activated signaling pathway in SCN\u2010projecting rat retinal ganglion cells"],"prefix":"10.1111","volume":"23","author":[{"given":"Erin J.","family":"Warren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles N.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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