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Whole\u2010cell voltage\u2010clamp recordings were made from rat SCN neurons to determine whether a retrograde messenger could modulate the activity of afferent \u03b3\u2010aminobutyric acid (GABA)ergic inputs. The frequency and amplitude of spontaneous GABAergic currents was significantly reduced in a subpopulation of SCN neurons (eight out of 13) following a postsynaptic depolarization. Similarly, a postsynaptic depolarization significantly reduced the amplitude of evoked GABAergic currents during both day and night recordings. A postsynaptic depolarizing pulse eliminated paired\u2010pulse inhibition of GABAergic currents consistent with a presynaptic mechanism. Muscimol\u2010activated currents were not altered by postsynaptic depolarization, demonstrating that the activity of GABA<jats:sub>A<\/jats:sub> receptors was not altered. Depolarization\u2010induced inhibition of the GABAergic currents was not observed when a Ca<jats:sup>2+<\/jats:sup> chelator was included in the microelectrode. Postsynaptic depolarization significantly increased the Ca<jats:sup>2+<\/jats:sup> concentration in both the soma and dendrites. The dendritic Ca<jats:sup>2+<\/jats:sup> levels increased faster, to a higher concentration and decayed faster than in the soma. The depolarization\u2010induced inhibition of the evoked GABAergic current was blocked by the G\u2010protein uncoupling agent N\u2010ethylmaleimide, suggesting that the retrograde messenger acts on a pertussis toxin\u2010sensitive G\u2010protein\u2010coupled receptor. Because the majority of SCN neurons receive GABAergic input from neighboring cells, these results describe a retrograde signaling mechanism by which SCN neurons can modulate GABAergic synaptic input.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.04850.x","type":"journal-article","created":{"date-parts":[[2006,6,19]],"date-time":"2006-06-19T13:41:31Z","timestamp":1150724491000},"page":"3209-3216","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Retrograde suppression of GABAergic currents in a subset of SCN neurons"],"prefix":"10.1111","volume":"23","author":[{"given":"Heinrich S.","family":"Gompf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert P.","family":"Irwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles N.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(02)00080-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00062-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-15-06373.2003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199601310-00040"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00241.2001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03382.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90361-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01082.2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873099129000515"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-02-00563.1991"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00164-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021917"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019416"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00314.2003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-20-j0005.2001"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00246-X"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00147.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.061bf.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80876-4"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(03)00072-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90120-A"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80983-6"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature728"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"4122","DOI":"10.1523\/JNEUROSCI.12-10-04122.1992","article-title":"Postsynaptic spike firing reduces synaptic GABAA responses in hippocampal pyramidal cells","volume":"12","author":"Pitler T.A.","year":"1992","journal-title":"J. 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