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The present study was designed to assess whether intrahippocampal injection of ET\u20101 in immature rats causes neurodegeneration and immediate seizures, and results in impairment of motor development, cognitive decline, epilepsy and chronic hippocampal lesion. ET\u20101 was injected unilaterally into the dorsal hippocampus in doses of 20 or 40\u2003pmol at the age of 12 (P12) or 25 (P25) days. Video\u2010electroencephalographic monitoring performed during 100\u2003min after the injection of ET\u20101 demonstrated the development of convulsive epileptic seizures in 75\u2013100% of animals of individual age\u2010and\u2010dose groups. Long\u2010term behavioral follow\u2010up did not reveal impairment of motor development in any dose\u2010and\u2010age group. At 2\u2003months after ET\u20101 injection, impairment of spatial memory occurred only in rats with 40\u2003pmol of ET\u20101 at P12. At 3\u2003months after ET\u20101 injection spontaneous electrographic seizures occurred in 62.5\u2013100% animals of both ages with no relation to the dose used. Seizures were always non\u2010convulsive. The total seizure duration per 24\u2003h was higher in the P12 than the P25 group, suggesting more severe epilepsy. The extent of the hippocampal lesion increased with the dose of ET\u20101 and was significantly higher in the P12 than the P25 group. The severity of the ET\u20101\u2010induced lesion correlated positively with total seizure duration per 24\u2003h at both ages. Our results document that early intrahippocampal injection of ET\u20101 results in lesion development and both immediate seizures and chronic epilepsy in either age group. Cognitive impairment occurred only in rats with ET\u20101 injection at P12.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.04910.x","type":"journal-article","created":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T13:17:09Z","timestamp":1152710229000},"page":"351-360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Intrahippocampal injection of endothelin\u20101 in immature rats results in neuronal death, development of epilepsy and behavioral abnormalities later in life"],"prefix":"10.1111","volume":"24","author":[{"given":"Ad\u00e9la","family":"M\u00e1t\u00e9ffyov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Ot\u00e1hal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grygoriy","family":"Tsenov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Mare\u0161","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Kubov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-3472(75)90114-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(02)02241-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00824337"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02959-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1995.tb01015.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(94)00332-D"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2004.04.010"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1152\/ajplegacy.1971.220.2.333","article-title":"Maturation of temperature homeostasis in the rat","volume":"22","author":"Conklin P.","year":"1971","journal-title":"Am. 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