{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:54:45Z","timestamp":1791039285906,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,8,14]],"date-time":"2006-08-14T00:00:00Z","timestamp":1155513600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sequences in the cytoplasmic II\u2013III loop of Ca<jats:sub>V<\/jats:sub>2 voltage\u2010gated calcium channels, termed the synaptic protein interaction (synprint) site, are considered important for the functional incorporation of presynaptic calcium channels into the synaptic vesicle fusion apparatus. Two novel Ca<jats:sub>V<\/jats:sub>2.2 splice variants lack large parts of the cytoplasmic II\u2010III loop (\u03941 R756\u2010L1139, \u03942 K737\u2010A1001) including the synprint protein\u2013protein interaction domain. Here we expressed green fluorescent protein (GFP)\u2010\u03b1<jats:sub>1B<\/jats:sub> subunit fusion constructs of Ca<jats:sub>V<\/jats:sub>2.2 splice variants in mouse hippocampal neurons to study their distribution in distinct neuronal compartments and to address the question of whether and how the synprint site functions in the presynaptic targeting of N\u2010type calcium channels. Similar to full\u2010length GFP\u2010\u03b1<jats:sub>1B<\/jats:sub> but divergent from the somatodendritic \u03b1<jats:sub>1C<\/jats:sub>\u2010HA (Ca<jats:sub>V<\/jats:sub>1.2) channel type, the splice variants GFP\u2010\u03b1<jats:sub>1B<\/jats:sub>\u2010\u03941 and GFP\u2010\u03b1<jats:sub>1B<\/jats:sub>\u2010\u03942 were targeted into the axons. Nevertheless, their ability to form <jats:italic>bona fide<\/jats:italic> presynaptic clusters was almost abolished for GFP\u2010\u03b1<jats:sub>1B<\/jats:sub>\u2010\u03941 and significantly reduced for GFP\u2010\u03b1<jats:sub>1B<\/jats:sub>\u2010\u03942. Thus, the synprint site is important for normal synaptic targeting of Ca<jats:sub>V<\/jats:sub>2.2 but not essential. Conversely, insertion of the synprint site into the II\u2013III loop of \u03b1<jats:sub>1C<\/jats:sub>\u2010HA did not restore axonal targeting or synaptic clustering. Together these results indicate that protein\u2013protein interactions with the synprint site must cooperate with other targeting mechanisms in the incorporation of Ca<jats:sub>V<\/jats:sub>2.2 into presynaptic specializations of hippocampal neurons but are neither necessary nor sufficient for axonal targeting. The unique targeting properties of the splice variants lacking the synprint site are suggestive of specific functions of these calcium channels apart from activating fast synaptic transmission.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.04947.x","type":"journal-article","created":{"date-parts":[[2006,8,14]],"date-time":"2006-08-14T12:06:16Z","timestamp":1155557176000},"page":"709-718","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Role of the synprint site in presynaptic targeting of the calcium channel Ca<sub>V<\/sub>2.2 in hippocampal neurons"],"prefix":"10.1111","volume":"24","author":[{"given":"Zsolt","family":"Szabo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald J.","family":"Obermair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conan 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