{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T06:26:22Z","timestamp":1769840782773,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,10,16]],"date-time":"2006-10-16T00:00:00Z","timestamp":1160956800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>5\u2010Hydroxytryptamine (5\u2010HT) inhibits transmitter release via activating GTP\u2010binding proteins, but the target of 5\u2010HT receptors in the nerve terminal is not determined. We addressed this question at the calyx of Held synapse in the brainstem slice of immature rats. Bath\u2010application of 5\u2010HT attenuated the amplitude of nerve\u2010evoked excitatory postsynaptic currents (EPSCs) associated with an increase in the paired\u2010pulse ratio, whereas it had no effect on the amplitude of spontaneous miniature EPSCs. The 5\u2010HT<jats:sub>1B<\/jats:sub> receptor agonist CP93129 mimicked the inhibitory effect of 5\u2010HT, but the 5\u2010HT<jats:sub>1A<\/jats:sub> agonist (R)\u2010(+)\u20108\u2010hydroxy\u2010DPAT (8\u2010OHDPAT) had no effect. The 5\u2010HT<jats:sub>1B<\/jats:sub> receptor antagonist NAS\u2010181 blocked the inhibitory effect of 5\u2010HT. These results suggest that 5\u2010HT activated 5\u2010HT<jats:sub>1B<\/jats:sub> receptors in calyceal nerve terminals, thereby inhibiting transmitter release. In direct whole\u2010cell recordings from calyceal nerve terminals, 5\u2010HT attenuated voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> currents, but had no effect on voltage\u2010dependent K<jats:sup>+<\/jats:sup> currents. When EPSCs were evoked by presynaptic Ca<jats:sup>2+<\/jats:sup> currents during simultaneous pre\u2010 and postsynaptic recordings, the magnitude of the 5\u2010HT\u2010induced inhibition of Ca<jats:sup>2+<\/jats:sup> currents fully explained that of EPSCs. Upon repetitive applications, 5\u2010HT showed tachyphylaxis, with its effect on both EPSCs and presynaptic Ca<jats:sup>2+<\/jats:sup> currents becoming weaker in the second application. 1,2\u2010bis(o\u2010aminophenoxy)ethane\u2010N\u2010N\u2032\u2010N\u2032\u2010N\u2032\u2010tetraacetic acid (BAPTA; 10\u2003m<jats:sc>m<\/jats:sc>) loaded into the nerve terminal abolished this tachyphylaxis. The presynaptic inhibitory effect of 5\u2010HT was prominent at postnatal day 5, but became weaker as animals matured. We conclude that activation of 5\u2010HT<jats:sub>1B<\/jats:sub> receptors inhibits voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels, thereby inhibiting transmitter release at immature calyceal nerve terminals, and that 5\u2010HT<jats:sub>1B<\/jats:sub> receptors undergo Ca<jats:sup>2+<\/jats:sup>\u2010dependent tachyphylaxis on repetitive activations.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.05063.x","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T09:27:56Z","timestamp":1161163676000},"page":"1946-1954","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["5\u2010HT<sub>1B<\/sub> receptor\u2010mediated presynaptic inhibition at the calyx of Held of immature rats"],"prefix":"10.1111","volume":"24","author":[{"given":"Haruo","family":"Mizutani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Hori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206686200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00498-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90098-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020974"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058803"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp021095"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017717"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019842"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"3130","DOI":"10.1523\/JNEUROSCI.23-08-03130.2003","article-title":"Presynaptic modulation of the retinogeniculate synapse","volume":"23","author":"Chen C.","year":"2003","journal-title":"J. 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