{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T02:20:15Z","timestamp":1789352415973,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2006,11,20]],"date-time":"2006-11-20T00:00:00Z","timestamp":1163980800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several lines of evidence suggest a close association between dopamine (DA) and neurotensin (NT) systems in the CNS. Indeed, in the rodent brain, abundant NT\u2010containing fibres are found in DA\u2010rich areas such as the ventral tegmental area and substantia nigra. Moreover, it has been shown<jats:italic>in vivo<\/jats:italic>that NT, acting through its high\u2010affinity receptor (NTS1), reduces the physiological and behavioural effects of DA D2 receptor (D2R) activation, a critical autoreceptor feedback system regulating DA neurotransmission. However, the mechanism of this interaction is still elusive. The aim of our study was thus to reproduce<jats:italic>in vitro<\/jats:italic>the interaction between D2R and NTS1, and then to characterize the mechanisms implicated. We used a primary culture model of DA neurons prepared from transgenic mice expressing green fluorescent protein under the control of the tyrosine hydroxylase promoter. In these cultures, DA neurons endogenously express both D2R and NTS1. Using electrophysiological recordings, we show that activation of D2R directly inhibits the firing rate of DA neurons. In addition, we find that NT, acting through a NTS1\u2010like receptor, is able to reduce D2R autoreceptor function independently of its ability to enhance DA neuron firing, and that this interaction occurs through a protein kinase C\u2010 and Ca<jats:sup>2+<\/jats:sup>\u2010dependent mechanism. Furthermore, prior activation of D2R reduces the ability of NTS1 to induce intracellular Ca<jats:sup>2+<\/jats:sup>mobilization. Our findings provide evidence for bidirectional interaction between D2R and NTS1 in DA neurons, a regulatory mechanism that could play a key role in the control of the activity of these neurons.<\/jats:p>","DOI":"10.1111\/j.1460-9568.2006.05151.x","type":"journal-article","created":{"date-parts":[[2006,11,20]],"date-time":"2006-11-20T16:19:14Z","timestamp":1164039554000},"page":"2789-2800","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Bidirectional regulation of dopamine D2 and neurotensin NTS1 receptors in dopamine neurons"],"prefix":"10.1111","volume":"24","author":[{"given":"C.","family":"Jomphe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.\u2010L.","family":"Lemelin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Okano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.\u2010E.","family":"Trudeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0031-6997(24)01509-6","article-title":"Neurotensin and dopamine interactions","volume":"53","author":"Binder E.B.","year":"2001","journal-title":"Pharmacol. 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