{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:54:17Z","timestamp":1791165257216,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T00:00:00Z","timestamp":1146700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Activity of extracellular acid phosphatases was measured at single\u2010cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake C\u0306ertovo, during the 2003 season, with a method based on use of the substrate ELF97 phosphate which provides fluorescent precipitates upon hydrolysis by phosphatases. The bacterial cell\u2010associated precipitates were quantified by image analysis. A specific, conspicuous, apparently homogeneous morphotype of curved cells of \u223c5\u2003\u00b5m average length, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity in Lake C\u0306ertovo (ranging from 1.0 to 12.7\u2003\u00b5mol\u2003l\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2003h\n                    <jats:sup>\u22121<\/jats:sup>\n                    , using ELF97 phosphate as a substrate). Bacterial filaments (&gt;\u200310\u2003\u00b5m), although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass\u2010specific activity of extracellular (cell\u2010surface) phosphatases of the main bacterioplankton morphotypes increased in the order filaments\u2003&lt;\u2003cocci and rods &lt;\u2003curved cells. The biomass\u2010specific activity of bacterioplankton extracellular phosphatases (0\u2013300\u2003nmol\u2003\u00b5gC\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2003h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) was generally highest in the spring and decreased gradually during summer. These changes could result from seasonal changes in the phosphorus status of the lake and from subsequent regulation of enzyme expression by bacteria.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2006.01023.x","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T05:33:33Z","timestamp":1146720813000},"page":"1271-1279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Specific activity of cell\u2010surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake"],"prefix":"10.1111","volume":"8","author":[{"given":"Jir\u0306\u00ed","family":"Nedoma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslav","family":"Vrba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,5,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps010257"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2001.46.1.0108"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1992.37.2.0232"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1984.29.2.0298"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2003.12.007"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8817.1973.tb04092.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame009105"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1995.40.7.1236"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps164021"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1127\/archiv-hydrobiol\/137\/1996\/265","article-title":"Measurement of phosphomonoesterase activity with a radiolabelled glucose\u20106\u2010phosphate. 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