{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:24:38Z","timestamp":1789867478006,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T00:00:00Z","timestamp":1169078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    We have recently developed a new personal sampler and demonstrated its feasibility for detection of viable airborne microorganisms including bacteria, fungi and viruses. To accelerate the time\u2010consuming analytical procedure involving 2\u20135\u2003days of biological testing, we employed a real\u2010time PCR protocol in conjunction with the personal sampler for collection of airborne viruses. The advantage of this approach is that if the presence of a particular pathogen in the air is detected by the PCR, the remaining collecting liquid can be further analysed by more time\u2010consuming biological methods to estimate the number of airborne infectious\/live microorganisms. As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted PCR analysis is required. Here we present the results of the study on the detection of\n                    <jats:italic>Influenza<\/jats:italic>\n                    virus in the ambient air contaminated with high concentrations of bacteria and fungi using real\u2010time PCR protocol. The combined sampling PCR detection method was found to be fully feasible for the rapid (\u223c2.5\u2003h) and highly specific (no cross\u2010reactivity) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2006.01226.x","type":"journal-article","created":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T13:57:33Z","timestamp":1169128653000},"page":"992-1000","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Using a bioaerosol personal sampler in combination with real\u2010time PCR analysis for rapid detection of airborne viruses"],"prefix":"10.1111","volume":"9","author":[{"given":"Oleg V.","family":"Pyankov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor E.","family":"Agranovski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Pyankova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Mokhonova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlad","family":"Mokhonov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander S.","family":"Safatov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander A.","family":"Khromykh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/027868201753227415"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1352-2310(01)00488-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atmosenv.2004.03.044"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.6963-6967.2004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaerosci.2004.11.014"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/02786820500297012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","unstructured":"Agranovski I. Safatov A. Sergeev A.A. Pyankov O. Petrishchenko V. Mikheev M.V. andSergeev A.N.(2006)Rapid detection of airborne viruses by personal bioaerosol sampler combined with the PCR device.Atmos Environ40:3924\u20133929.","DOI":"10.1016\/j.atmosenv.2006.02.023"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90005-5"},{"key":"e_1_2_7_10_1","first-page":"119","volume-title":"Virology: A Practical Approach","author":"Barrett T.","year":"1985"},{"key":"e_1_2_7_11_1","first-page":"37","volume-title":"PCR Primer: A Laboratory Manual","author":"Cha R.S.","year":"1995"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007606603553"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4302-4308.2001"},{"key":"e_1_2_7_14_1","volume-title":"Standard Methods for the Examination of Water and Wastewater","author":"Greenberg A.E.","year":"1992"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1128\/JCM.37.3.828-829.1999","article-title":"Unexpected cross\u2010reaction with Fusobacterium necrophorum in a PCR for detection of mycoplasmas","volume":"37","author":"Jensen J.S.","year":"1999","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaerosci.2005.02.004"},{"key":"e_1_2_7_17_1","volume-title":"Concepts and Procedures for Laboratory\u2010Based Influenza Surveillance","author":"Kendal A.P.","year":"1982"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-8502(94)90215-1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086616"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/1047322X.1997.10387755"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","first-page":"3735","DOI":"10.1128\/JCM.38.10.3735-3742.2000","article-title":"Development of a PCR\u2010based line probe assay for identification of fungal pathogens","volume":"38","author":"Martin C.","year":"2000","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2004.06.027"},{"key":"e_1_2_7_23_1","first-page":"493","article-title":"A simple method for estimating fifty percent end points","volume":"27","author":"Reed L.J.","year":"1938","journal-title":"Am J Hyg"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1462-2920.2006.01226.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1462-2920.2006.01226.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T21:37:38Z","timestamp":1761860258000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1462-2920.2006.01226.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,18]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1111\/j.1462-2920.2006.01226.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1462-2920.2006.01226.x","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,18]]},"assertion":[{"value":"2007-01-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}