{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:01:03Z","timestamp":1791252063442,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T00:00:00Z","timestamp":1169078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"Summary<\/jats:title>\n \n Legionella pneumophila<\/jats:italic>\n , the causative agent of Legionnaires' disease, is ubiquitously found in aquatic environments, associated with free living amoebae. Trophozoite forms of the genus\n Acanthamoeba<\/jats:italic>\n have been shown to support the intracellular growth of\n Legionella<\/jats:italic>\n while it has been proposed that cyst forms are related to survival in harsh environments. This underlines that amoebae are of primary importance in\n Legionella<\/jats:italic>\n spreading. In this study, we followed the survival of\n L.\u2003pneumophila<\/jats:italic>\n Lens over 6\u2003months in a poor medium, with or without\n Acanthamoeba castellanii<\/jats:italic>\n . The results demonstrated that\n L.\u2003pneumophila<\/jats:italic>\n Lens could survive for at least 6\u2003months in association with\n A.\u2003castellanii<\/jats:italic>\n and that cultivable bacteria are to be found within expelled vesicles rather than within cysts. Our findings suggest that vesicles might be further studied in order to elucidate their production and their role in the environmental spreading of\n Legionella<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/j.1462-2920.2006.01229.x","type":"journal-article","created":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T13:57:33Z","timestamp":1169128653000},"page":"1341-1344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["Long\u2010term survival of\n Legionella pneumophila<\/i>\n associated with\n Acanthamoeba castellanii<\/i>\n vesicles"],"prefix":"10.1111","volume":"9","author":[{"given":"Sabrina","family":"Bouyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Imbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010H\u00e9l\u00e8ne","family":"Rodier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"H\u00e9chard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,18]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.1.600-606.2003"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400060174"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.1.279-286.1998"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.3.1562-1569.2005"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094226"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.24.7025-7041.2002"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2003.08.003"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.2.413-433.2004"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb02904.x"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.273-307.2003"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.12.1179"},{"key":"e_1_2_4_14_1","first-page":"678","article-title":"Current views on the relationships between amoebae, legionellae and man","volume":"22","author":"Rowbotham T.J.","year":"1986","journal-title":"Isr J Med Sci"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-444601-4.50015-1"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)90277-5"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.6.2256-2261.1998"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00607.x"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00703.x"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1462-2920.2006.01229.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1462-2920.2006.01229.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T05:42:12Z","timestamp":1761889332000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1462-2920.2006.01229.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,18]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1111\/j.1462-2920.2006.01229.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1462-2920.2006.01229.x","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,18]]},"assertion":[{"value":"2007-01-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}