{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:07:43Z","timestamp":1791205663733,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T00:00:00Z","timestamp":1313712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Dichloromethane (CH\n                    <jats:sub>2<\/jats:sub>\n                    Cl\n                    <jats:sub>2<\/jats:sub>\n                    , DCM) is a chlorinated solvent mainly produced by industry, and a common pollutant. Some aerobic methylotrophic bacteria are able to grow with this chlorinated methane as their sole carbon and energy source, using a DCM dehalogenase\/glutathione\n                    <jats:italic>S<\/jats:italic>\n                    \u2010transferase encoded by\n                    <jats:italic>dcmA<\/jats:italic>\n                    to transform DCM into two molecules of HCl and one molecule of formaldehyde, a toxic intermediate of methylotrophic metabolism. In\n                    <jats:italic>Methylobacterium extorquens<\/jats:italic>\n                    DM4 of known genome sequence,\n                    <jats:italic>dcmA<\/jats:italic>\n                    lies on a 126\u2003kb\n                    <jats:italic>dcm<\/jats:italic>\n                    genomic island not found so far in other DCM\u2010dechlorinating strains. An experimental search for the molecular determinants involved in specific cellular responses of strain DM4 growing with DCM was performed. Random mutagenesis with a minitransposon containing a promoterless reporter\n                    <jats:italic>gfp<\/jats:italic>\n                    gene yielded 25\n                    <jats:italic>dcm<\/jats:italic>\n                    mutants with a specific DCM\u2010associated phenotype. Differential proteomic analysis of cultures grown with DCM and with methanol defined 38 differentially abundant proteins. The 5.5\u2003kb\n                    <jats:italic>dcm<\/jats:italic>\n                    islet directly involved in DCM dehalogenation is the only one of seven gene clusters specific to the DCM response to be localized within the\n                    <jats:italic>dcm<\/jats:italic>\n                    genomic island. The DCM response was shown to involve mainly the core genome of\n                    <jats:italic>Methylobacterium extorquens<\/jats:italic>\n                    , providing new insights on DCM\u2010dependent adjustments of C1 metabolism and gene regulation, and suggesting a specific stress response of\n                    <jats:italic>Methylobacterium<\/jats:italic>\n                    during growth with DCM. Fatty acid, hopanoid and peptidoglycan metabolisms were affected, hinting at the membrane\u2010active effects of DCM due to its solvent properties. A chloride\u2010induced efflux transporter termed CliABC was also newly identified. Thus, DCM dechlorination driven by the\n                    <jats:italic>dcm<\/jats:italic>\n                    islet elicits a complex adaptive response encoded by the core genome common to dechlorinating as well as non\u2010dechlorinating\n                    <jats:italic>Methylobacterium<\/jats:italic>\n                    strains.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2011.02524.x","type":"journal-article","created":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T16:43:19Z","timestamp":1313772199000},"page":"2518-2535","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Functional genomics of dichloromethane utilization in\n                    <i>Methylobacterium extorquens<\/i>\n                    DM4"],"prefix":"10.1111","volume":"13","author":[{"given":"Emilie E. 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