{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:11:49Z","timestamp":1768263109511,"version":"3.49.0"},"reference-count":70,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T00:00:00Z","timestamp":1312416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In biofilters of recirculation aquaculture systems (RAS), nitrification by lithoautotrophic microorganisms is essential to prevent the cultivated organisms from intoxication with ammonium and nitrite. In moving\u2010bed biofilters nitrifying microorganisms are immobilized together with heterotrophic bacteria in dense biofilms on carrier elements like plastic beads. Analyses of fatty acid profiles of these biofilms from a marine biofilter revealed a high abundance of\n                    <jats:italic>Nitrospira<\/jats:italic>\n                    \u2010related lipid markers (8\u201312% of total fatty acids). Further results of a labeling experiment with\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010bicarbonate in mineral salts medium with 3\u2003mM nitrite confirmed that\n                    <jats:italic>Nitrospira<\/jats:italic>\n                    is the major autotrophic nitrite oxidizer in the biofilter system. According to 16S rRNA gene sequence analyses the nitrite\u2010oxidizing community in the biofilter consisted of at least two different representatives of\n                    <jats:italic>Nitrospira<\/jats:italic>\n                    , one of which could be successfully isolated. The marine isolate \u2018Ecomares 2.1\u2019 belongs to cluster IVa and showed 98.8% 16S rRNA gene sequence similarity to\n                    <jats:italic>Nitrospira marina,<\/jats:italic>\n                    whereas the enrichment \u2018M1 marine\u2019 is only distantly related (94.0% 16S rRNA gene sequence similarity to\n                    <jats:italic>N. marina<\/jats:italic>\n                    ). In laboratory experiments, the isolate exhibited remarkable tolerances against high substrate and product concentrations (30\u2003mM nitrite and 80\u2003mM nitrate) as well as ammonium (50\u2003mM). During the isolation process a strong tendency of this strain to develop biofilms became apparent. Thus, Ecomares 2.1 seems to be well adapted to the attached lifestyle in biofilters and the nitrogenous load prevailing in the effluent waters of RAS. Both members of\n                    <jats:italic>Nitrospira<\/jats:italic>\n                    could be detected by PCR\u2010based methods in environmental samples of marine and brackish RAS biofilters and are therefore considered to be characteristic for these engineered ecosystems.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2011.02525.x","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T11:00:30Z","timestamp":1312455630000},"page":"2536-2547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Relevance of\n                    <i>Nitrospira<\/i>\n                    for nitrite oxidation in a marine recirculation aquaculture system and physiological features of a\n                    <i>Nitrospira marina<\/i>\n                    \u2010like isolate"],"prefix":"10.1111","volume":"13","author":[{"given":"Sabine","family":"Keuter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Kruse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Lipski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Spieck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,4]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Alawi M.(2007)Diversit\u00e4t Nitrit oxidierender Bakterien in B\u00f6den des nordsibirischen Permafrostes und Sedimenten der Laptev\u2010See. Dissertation. Hamburg Germany: University of Hamburg."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.34"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.7724-7736.2005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.9.4126-4133.1999"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2007.01.043"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0850440"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/33900"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2004.10.044"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl889"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.5273-5284.2001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02568729"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2007.00418.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506625102"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.45"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2010.01.090"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.59"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.1.258-264.1998"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.02.037"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2007.01.007"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.8.3042-3051.1998"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2005.12.023"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2005.03.032"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2003.00510.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame046177"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9320-4"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425275"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65379-0"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8659(1986)115<183:TONTF>2.0.CO;2"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80014-8"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1078\/0723-2020-00049"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0144-8609(00)00048-0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003860107"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01033.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80121-9"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00245215"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04378.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01053.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(90)90270-W"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21004"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00320-10"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.3182-3191.1999"},{"key":"e_1_2_7_45_1","first-page":"1470","article-title":"Nitrospira community composition in nitrifying reactors operated with two different dissolved oxygen levels","volume":"18","author":"Park H.D.","year":"2008","journal-title":"J Microbiol Biotechnol"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.1.208"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaeng.2005.04.002"},{"key":"e_1_2_7_48_1","first-page":"199","volume-title":"Methods on Phytobacteriology","author":"Sasser M.","year":"1990"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409123"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3480-3485.1998"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2000.00150.x"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1139\/s05-044"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-381294-0.00005-5"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0076"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90033-1"},{"key":"e_1_2_7_56_1","first-page":"152","article-title":"Taxonomic parameters revisited: tarnished gold standards","volume":"33","author":"Stackebrandt E.","year":"2006","journal-title":"Microbiol Today"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.3.2050-2063.2006"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/22749"},{"key":"e_1_2_7_59_1","first-page":"2301","article-title":"Nitrification in fixed\u2010bed reactors treating saline wastewater","volume":"85","author":"Sudarno U.","year":"2009","journal-title":"Appl Microbiol Biotechnol"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2004.09.028"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(02)00372-1"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2896-2904.2006"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100766"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/br.41.1.100-180.1977"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(96)00376-4"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(99)00175-X"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.50.2.194-201.1985"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.4.4.475"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00454947"},{"key":"e_1_2_7_71_1","first-page":"1808","volume-title":"Bergey's Manual of Systematic Bacteriology","author":"Watson S.W.","year":"1989"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1462-2920.2011.02525.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1462-2920.2011.02525.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T05:57:52Z","timestamp":1761890272000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1462-2920.2011.02525.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8,4]]},"references-count":70,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2011,9]]}},"alternative-id":["10.1111\/j.1462-2920.2011.02525.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1462-2920.2011.02525.x","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8,4]]},"assertion":[{"value":"2011-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}