{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T06:39:50Z","timestamp":1776407990291,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T00:00:00Z","timestamp":1315267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Eimeria gruis<\/jats:italic>\n                    and\n                    <jats:italic>E.\u2003reichenowi<\/jats:italic>\n                    cause coccidiosis, a major parasitic disease of cranes. By non\u2010invasive molecular approaches, we investigated the prevalence and genetic characterization of pathogens in two Japanese crane habitats; one is Hokkaido inhabited by the endangered red\u2010crowned crane, and the other is Izumi in Kyushu where populations that consist mainly of vulnerable hooded and white\u2010naped cranes migrate in winter. The non\u2010invasively collected faecal samples from each wintering population were first subjected to host genomic DNA\u2010targeted analyses to determine the sample origin and avoid sample redundancy. Extremely high prevalence was observed in the Izumi populations (&gt;\u200390%) compared with the Hokkaido population (18\u201330%) by examining 470 specimens by microscopy and PCR\u2010based capillary electrophoresis (PCR\u2010CE), using genetic markers in the second internal transcribed spacer (ITS2). Correspondence analysis of PCR\u2010CE data revealed differences in community composition of coccidia between hooded and white\u2010naped cranes. 18S rRNA and ITS2 sequences were determined from single oocysts excreted by red\u2010crowned and hooded cranes. Phylogenetic analysis of 18S rRNA suggested that\n                    <jats:italic>E.\u2003reichenowi<\/jats:italic>\n                    was polyphyletic while\n                    <jats:italic>E.\u2003gruis<\/jats:italic>\n                    was monophyletic. Together with PCR\u2010CE data, these results indicate different host specificity among the\n                    <jats:italic>E.\u2003reichenowi<\/jats:italic>\n                    type. Our data suggest that\n                    <jats:italic>E.\u2003reichenowi<\/jats:italic>\n                    comprises multiple species.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2011.02563.x","type":"journal-article","created":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T02:32:34Z","timestamp":1315276354000},"page":"2876-2887","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Accurate analysis of prevalence of coccidiosis in individually identified wild cranes in inhabiting and migrating populations in Japan"],"prefix":"10.1111","volume":"13","author":[{"given":"Hajime","family":"Honma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Suyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Nakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.10.3946-3949.1997"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-003-1345-x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/3279468"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001144"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/92.5.392"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284470"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2007.02.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284150"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500103"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.16.685"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.2000.01042-11.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00809-08"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0365-y"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03048.x"},{"key":"e_1_2_7_17_1","unstructured":"IUCN2010(2010)IUCN Red List of Threatened Species. 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