{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:01:10Z","timestamp":1791129670364,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T00:00:00Z","timestamp":1320278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Bacterial wilt (brown rot) disease of potato caused by\n                    <jats:italic>Ralstonia solanacearum<\/jats:italic>\n                    is one of the most important bacterial diseases and a major constraint on potato production worldwide. Through a comparative genomic analysis between\n                    <jats:italic>R.\u2003solanacearum<\/jats:italic>\n                    \u2018race 3 biovar 2\u2019 (R3bv2) strains, we identified a 77\u2003kb region in strain UW551 which is specifically absent in the hypoaggressive strain IPO1609. We proved that IPO1609 indeed carries a 77\u2003kb genomic deletion and provide genetic evidence that occurrence of this deletion is responsible for almost complete loss of pathogenicity of this strain. We carried out a functional analysis of this 77\u2003kb region in strain UW551 using a combination of gene deletion and functional complementation approaches which identified the methionine biosynthesis genes\n                    <jats:italic>metER<\/jats:italic>\n                    as having a major contribution to IPO1609 pathogenesis. Deletion of the\n                    <jats:italic>metER<\/jats:italic>\n                    genes significantly impacts pathogenicity of R3bv2 strains but does not lead to methionine auxotrophy nor reduced ability to multiply\n                    <jats:italic>in planta<\/jats:italic>\n                    . In addition, this study indicated that three type III secretion system effectors or a type VI secretion system present within the 77\u2003kb region have no or very minor contribution to pathogenicity.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2011.02636.x","type":"journal-article","created":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T06:22:52Z","timestamp":1320301372000},"page":"3172-3185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Detection and functional characterization of a large genomic deletion resulting in decreased pathogenicity in\n                    <i>Ralstonia solanacearum<\/i>\n                    race 3 biovar 2 strains"],"prefix":"10.1111","volume":"13","author":[{"given":"Adriana","family":"Gonz\u00e1lez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Plener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Boucher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Genin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-98-1-0059"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509393103"},{"key":"e_1_2_5_4_1","volume-title":"Short Protocols in Molecular Biology","author":"Ausubel F.","year":"1995"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00430438"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2002.15.2.109"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.12.4407"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.131"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01253-06"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/m74-080"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04118.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-4538-7_16"},{"key":"e_1_2_5_13_1","first-page":"9","volume-title":"Bacterial Wilt Disease and the Ralstonia Solanacearum Species Complex","author":"Elphinstone J.G.","year":"2005"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2000.90.12.1358"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001055"},{"key":"e_1_2_5_16_1","first-page":"449","volume-title":"Bacterial Wilt Disease and the Ralstonia Solanacearum Species Complex","author":"Fegan M.","year":"2005"},{"key":"e_1_2_5_17_1","first-page":"163","volume-title":"Microbiol. 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