{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:28:27Z","timestamp":1790141307022,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T00:00:00Z","timestamp":1344902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The catalase family of\n                    <jats:italic>Beauveria bassiana<\/jats:italic>\n                    (fungal entomopathogen) consists of catA (spore\u2010specific), catB (secreted), catP (peroxisomal), catC (cytoplasmic) and catD (secreted peroxidase\/catalase), which were distinguished in phylogeny and structure and functionally characterized by constructing single\u2010gene disrupted and rescued mutants for enzymatic and multi\u2010phenotypic analyses. Total catalase activity decreased 89% and 56% in \u0394\n                    <jats:italic>catB<\/jats:italic>\n                    and \u0394\n                    <jats:italic>catP<\/jats:italic>\n                    , corresponding to the losses of upper and lower active bands gel\u2010profiled for all catalases respectively, but only 9\u221212% in other knockout mutants. Compared with wild type and complement mutants sharing similar enzymatic and phenotypic parameters, all knockout mutants showed significant (9\u221256%) decreases in the antioxidant capability of their conidia (active ingredients of mycoinsecticides), followed by remarkable phenotypic defects associated with the fungal biocontrol potential. These defects included mainly the losses of 40% thermotolerance (45\u00b0C) in \u0394\n                    <jats:italic>catA<\/jats:italic>\n                    , 46\u221248% UV\u2010B resistance in \u0394\n                    <jats:italic>catA<\/jats:italic>\n                    and \u0394\n                    <jats:italic>catD<\/jats:italic>\n                    , and 33\u221247% virulence to\n                    <jats:italic>Spodoptera litura<\/jats:italic>\n                    larvae in \u0394\n                    <jats:italic>catA<\/jats:italic>\n                    , \u0394\n                    <jats:italic>catP<\/jats:italic>\n                    and \u0394\n                    <jats:italic>catD<\/jats:italic>\n                    respectively. Moreover, the drastic transcript upregulation of some other catalase genes observed in the normal culture of each knockout mutant revealed functionally complimentary effects among some of the catalase genes, particularly between\n                    <jats:italic>catB<\/jats:italic>\n                    and\n                    <jats:italic>catC<\/jats:italic>\n                    whose knockout mutants displayed little or minor phenotypic changes. However, the five catalase genes functioned redundantly in mediating the fungal tolerance to either hyperosmotic or fungicidal stress. The differentiated roles of five catalases in regulating the\n                    <jats:italic>B.\u2003bassiana<\/jats:italic>\n                    virulence and tolerances to oxidative stress, high temperature and UV\u2010B irradiation provide new insights into fungal adaptation to stressful environment and host invasion.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2012.02848.x","type":"journal-article","created":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T04:58:55Z","timestamp":1343192335000},"page":"409-418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":131,"title":["Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental 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