{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:08:27Z","timestamp":1790903307728,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,8,4]],"date-time":"2006-08-04T00:00:00Z","timestamp":1154649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:p>\n                    <jats:bold>Aim:\u2002<\/jats:bold>\n                    The novel adipokine visfatin has \u2018insulin\u2010mimicking\u2019 effects and is increased in models of diet\u2010induced obesity, but factors that regulate visfatin have not been fully elucidated.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:\u2002<\/jats:bold>\n                    In order to determine visfatin regulation in adipocyte development and metabolism, as well as in pathophysiological conditions related to metabolic syndrome, endogenous visfatin expression was measured in 3T3\u2010L1 pre\u2010adipocytes and adipocytes using real\u2010time reverse transcriptase polymerase chain reaction (real\u2010time RT\u2010PCR).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    A marked increase in visfatin expression was observed during differentiation, with a 2.2\u2010fold increase between preconfluent and 2\u2010day confluent cells even before differentiation was initiated. A further 4.1\u2010fold increase was induced from day 0 to day 9 of differentiation (overall ninefold). Overnight incubation with dexamethasone (10\n                    <jats:sup>\u22128<\/jats:sup>\n                    to 10\n                    <jats:sup>\u22122<\/jats:sup>\n                    M) increased visfatin expression in both pre\u2010adipocytes (1.5\u2010 to 3.3\u2010fold, p &lt; 0.05) and adipocytes (1.9\u2010fold, p &lt; 0.01). All other treatments decreased visfatin expression. In pre\u2010adipocytes, visfatin expression decreased by 23% at a concentration of 1 \u03bcM insulin, 15% at 1\u201315 nM T3, 15% at 10 nM\u20131 \u03bcM progesterone, 33\u201344% at 10 nM\u20131 \u03bcM testosterone, 50% with palmitate and 30% with oleate (p &lt; 0.05 for all). In adipocytes, insulin had a much greater effect, decreasing visfatin by 77% at 100 nM (p &lt; 0.01), whereas oleate and sex hormones did not affect visfatin expression. However, tumor necrosis factor \u03b1, which had no effect on pre\u2010adipocytes, significantly decreased visfatin in adipocytes by 26% at 10 ng\/ml (p &lt; 0.05). Interestingly, the thiazolidinedione (TZD) rosiglitizone also decreased visfatin by 28% at a concentration of 1 \u03bcM (p &lt; 0.01).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:\u2002<\/jats:bold>\n                    In summary, while the mechanism of visfatin action remains to be elucidated, the clear effects of multiple hormones on visfatin expression support a physiological role.\n                  <\/jats:p>","DOI":"10.1111\/j.1463-1326.2006.00625.x","type":"journal-article","created":{"date-parts":[[2006,8,4]],"date-time":"2006-08-04T21:07:44Z","timestamp":1154725664000},"page":"490-497","source":"Crossref","is-referenced-by-count":57,"title":["Visfatin expression is hormonally regulated by metabolic and sex hormones in 3T3\u2010L1 pre\u2010adipocytes and adipocytes"],"prefix":"10.1111","volume":"9","author":[{"given":"R.","family":"MacLaren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Cianflone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1097243"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1139\/o03-078"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041213"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2003.56"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-005-1505-2"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(01)00097-9"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1106933"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.05.058"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.10.2911"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17911"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1995.tb01532.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00686-7"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(05)70010-8"},{"key":"e_1_2_6_15_2","first-page":"523","article-title":"Insulin 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