{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:41:50Z","timestamp":1768315310086,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,5,24]],"date-time":"2011-05-24T00:00:00Z","timestamp":1306195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>\n                    <jats:bold>Aim:<\/jats:bold>\n                    Delayed graft revascularization impedes the success of human islet transplantation. This study utilized rotational co\u2010culture of insulin secreting\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010cells with human umbilical vein endothelial cells (HUVECs) and a peroxisome proliferator\u2010activated receptor gamma (PPAR\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    ) agonist to promote insulin and vascular endothelial growth factor (VEGF) secretory function.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Clonal BRIN\u2010BD11 (D11) cells were maintained in static culture (SC) and rotational culture (RC) \u00b1 HUVEC and \u00b1 the TZD (thiazolidinedione) rosiglitazone (10 mmol\/l) as a specific PPAR\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    agonist. HUVECs were cultured in SC and RC \u00b1 D11 and \u00b1 TZD. D11 insulin secretion was induced by static incubation with low glucose (1.67 mmol\/l), high glucose (16.7 mmol\/l) and high glucose with 10 mmol\/l theophylline (G+T) and assessed by enzyme\u2010linked immunosorbent assay (ELISA). HUVEC proliferation was determined by ATP luminescence, whereas VEGF secretion was quantified by ELISA. Co\u2010cultured cells were characterized by immunostaining for insulin and CD31.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    D11 SC and RC showed enhanced insulin secretion in response to 16.7 mmol\/l and G+T (p &lt; 0.01); without significant alteration by the TZD. Co\u2010culture with HUVEC in SC and RC also increased D11 insulin secretion when challenged with 16.7 mmol\/l and G+T (p &lt; 0.01), and this was slightly enhanced by the TZD. The presence of HUVEC increased D11 SC and RC insulin secretion in response to high glucose and G+T, respectively (p &lt; 0.01). Addition of the TZD increased SC and RC HUVEC ATP content (p &lt; 0.01) and VEGF production (p &lt; 0.01) in the presence and absence of D11 cells.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Rotational co\u2010culture of insulin secreting cells with endothelial cells, and exposure to a PPAR\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    agonist may improve the prospects for graft revascularization and function after implantation.\n                  <\/jats:p>","DOI":"10.1111\/j.1463-1326.2011.01392.x","type":"journal-article","created":{"date-parts":[[2011,3,16]],"date-time":"2011-03-16T13:34:26Z","timestamp":1300282466000},"page":"662-668","source":"Crossref","is-referenced-by-count":19,"title":["Rotational co\u2010culture of clonal\n                    <i>\u03b2<\/i>\n                    \u2010cells with endothelial cells: effect of PPAR\u2010\n                    <i>\u03b3<\/i>\n                    agonism\n                    <i>in vitro<\/i>\n                    on insulin and VEGF secretion"],"prefix":"10.1111","volume":"13","author":[{"given":"M. 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