{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T04:43:36Z","timestamp":1774327416589,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T00:00:00Z","timestamp":1334707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>\n                    <jats:bold>Aim:<\/jats:bold>\n                    Exercise and insulin each increase microvascular blood flow and enhance glucose disposal in skeletal muscle. We have reported that insulin\u2010mediated microvascular recruitment in a diet\u2010induced model of insulin resistance (high\u2010fat feeding for 4 weeks) is markedly impaired; however, the effect of muscle contraction in this model has not been previously explored.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    We fed rats either normal (ND, 10% calories from fat) or high\u2010fat (HFD, 60% calories from fat) diets\n                    <jats:italic>ad libitum<\/jats:italic>\n                    for 4\u20138 weeks. Animals were then anaesthetized and one hindlimb electrically stimulated to contract at 0.05, 0.1 and 2 Hz (field stimulation, 30 V, 0.1 ms duration) in 15 min stepwise increments. Femoral artery blood flow (Transonic flow probe), muscle microvascular blood flow (hindleg metabolism of 1\u2010methylxanthine and contrast\u2010enhanced ultrasound) and muscle glucose disposal (uptake of radiolabelled 2\u2010deoxy\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucose and hindleg glucose disappearance) were measured.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Both ND and HFD rats received the same voltage across the leg and consequently developed the same muscle tension. Femoral artery blood flow in the contracting leg increased during 2 Hz contraction, but not during the lower frequencies and these effects were similar between ND and HFD rats. Muscle microvascular blood flow significantly increased in a contraction frequency\u2010dependent manner, and preceded increases in total limb blood flow and these effects were similar between ND and HFD rats. Muscle glucose disposal was markedly elevated during 2 Hz contraction and was comparable between ND and HFD rats.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Contraction\u2010mediated muscle microvascular recruitment and glucose uptake are not impaired in the HFD insulin resistant rat.\n                  <\/jats:p>","DOI":"10.1111\/j.1463-1326.2012.01598.x","type":"journal-article","created":{"date-parts":[[2012,3,19]],"date-time":"2012-03-19T13:55:55Z","timestamp":1332165355000},"page":"753-761","source":"Crossref","is-referenced-by-count":16,"title":["Microvascular blood flow responses to muscle contraction are not altered by high\u2010fat feeding in rats"],"prefix":"10.1111","volume":"14","author":[{"given":"P.","family":"St\u2010Pierre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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