{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:57:32Z","timestamp":1787014652779,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,11]],"date-time":"2005-01-11T00:00:00Z","timestamp":1105401600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Samples were collected from 36 cats with feline herpesvirus (FHV\u20101)\u2010related ocular disease (conjunctivitis, epithelial or stromal keratitis, or corneal sequestration), and 17 cats without ocular changes. Corneoconjunctival swabs, scrapings and biopsies were tested in various combinations for presence of FHV\u20101 DNA using single round (sr) polymerase chain reaction (PCR) and nested PCR (nPCR). Additional swabs from the inferior conjunctival fornix were tested by enzyme\u2010linked immunosorbent assay for\n                    <jats:italic>Chlamydophila felis<\/jats:italic>\n                    antigen. Cytologic evaluation was carried out on conjunctival (cats with conjunctivitis) and corneal (cats with keratitis) cytobrush preparations. FHV\u20101 DNA was detected by PCR in 14 (39%) cats with ocular disease and 1 (6%) of the control group. Agreement between srPCR and nPCR results was significant (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). FHV\u20101 DNA was detected in 3\/7 cats with conjunctivitis, 5\/6 cats with epithelial keratitis, 3\/11 cats with stromal keratitis, and 3\/12 cats with corneal sequestration. There was a significant association (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.0027) between viral presence and epithelial keratitis. However, no significant association was found between viral presence and conjunctivitis (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.059), stromal keratitis (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.15), or corneal sequestration (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.18). With respect to FHV\u20101 DNA detection, intersample agreement was significant (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.03). No sampling technique seemed more likely than another to harvest detectable viral DNA, except for cats with corneal sequestrum in which viral DNA was not detected using corneoconjunctival swabs. FHV\u20101 DNA was detected in 6\/9 samples with intranuclear inclusion bodies and in 6\/7 cats with eosinophils on cytologic examination. All samples tested negative for\n                    <jats:italic>C. felis<\/jats:italic>\n                    antigen.\n                  <\/jats:p>","DOI":"10.1111\/j.1463-5224.2005.04060.x","type":"journal-article","created":{"date-parts":[[2005,1,11]],"date-time":"2005-01-11T13:46:07Z","timestamp":1105451167000},"page":"25-32","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Cytologic findings, and feline herpesvirus DNA and\n                    <i>Chlamydophila felis<\/i>\n                    antigen detection rates in normal cats and cats with conjunctival and corneal lesions"],"prefix":"10.1111","volume":"8","author":[{"given":"Sabine","family":"Volopich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane","family":"Benetka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Schwendenwein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"M\u00f6stl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Sommerfeld\u2010Stur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Nell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,11]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-97-23783"},{"key":"e_1_2_5_3_2","first-page":"1758","article-title":"Experimental 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