{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T07:05:52Z","timestamp":1793171152421,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,11]],"date-time":"2005-01-11T00:00:00Z","timestamp":1105401600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold>The ocular microflora in dogs has not been established in north\u2010east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Cear\u00e1, Brazil.<\/jats:p><jats:p><jats:bold>Animals studied\u2002<\/jats:bold>This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers.<\/jats:p><jats:p><jats:bold>Procedure\u2002<\/jats:bold>Samples were taken by a calibrated platinum loop (1\u00a0\u00b5L) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37\u00a0\u00b0C in an atmosphere of 5% CO<jats:sub>2<\/jats:sub>for 48\u00a0h.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold>Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) number of colony\u2010forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram\u2010positive bacteria (86.5%) predominated over Gram\u2010negative (13.5%).<jats:italic>Staphylococcus<\/jats:italic>spp. was the most frequently isolated genus and<jats:italic>S. intermedius<\/jats:italic>predominated in both groups.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold>The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer.<\/jats:p>","DOI":"10.1111\/j.1463-5224.2005.04061.x","type":"journal-article","created":{"date-parts":[[2005,1,11]],"date-time":"2005-01-11T18:46:07Z","timestamp":1105469167000},"page":"33-37","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Cear\u00e1, Brazil"],"prefix":"10.1111","volume":"8","author":[{"given":"Marilena R.","family":"Prado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos F. 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